Application
Fig1: Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti-ROR Gamma (ER1916-09) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 57 kDApplication
Fig2: Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti-ROR Gamma (ER1916-09) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 57 kDApplication
Fig3: Blank control:Molt-4. Primary Antibody (green line): Rabbit Anti-ROR Gamma antibody (ER1916-09) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Application
Fig4: Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (RORG) Polyclonal Antibody, Unconjugated (ER1916-09) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | ROR Gamma |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RORG NR1F3 Nuclear receptor ROR gamma Nuclear receptor ROR-gamma Nuclear receptor RZR gamma Nuclear receptor RZR-gamma Nuclear receptor subfamily 1 group F member 3 RAR related orphan receptor C RAR related orphan receptor C, isoform a RAR related orphan receptor gamma Retinoic acid binding receptor gamma Retinoid related orphan receptor gamma Retinoid-related orphan receptor-gamma Rorc RORG_HUMAN RZR GAMMA RZRG TOR. |
| Product description | Possible nuclear receptor for hydroxycholesterols, the binding of which strongly promotes coactivators recruitment. Essential for thymopoiesis and the development of several secondary lymphoid tissues, including lymph nodes. Involved in lineage specification of uncommitted CD4(+) T helper cells into Th17 cells. Regulate the expression of several components of the circadian clock. |
| Immunogen | KLH conjugated synthetic peptide derived from human RORG 415-518/518 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:1ug/test: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorse |
| Concentration | 1mg/ml |
| Alternative Names | RORG
NR1F3
Nuclear receptor ROR gamma
Nuclear receptor ROR-gamma
Nuclear receptor RZR gamma
Nuclear receptor RZR-gamma
Nuclear receptor subfamily 1 group F member 3
RAR related orphan receptor C
RAR related orphan receptor C isoform a RAR related orphan receptor gamma Retinoic acid binding receptor gamma Retinoid related orphan receptor gamma Retinoid-related orphan receptor-gamma Rorc RORG_HUMAN RZR GAMMA RZRG TOR. |
|---|---|
| Molecular Weight(MW) | 57 KDa |
| Cellular Localization | Nucleus (Probable). |
| SwissProt ID | P51449 |
|---|
Application
Fig1: Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti-ROR Gamma (ER1916-09) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 57 kD
Application
Fig2: Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti-ROR Gamma (ER1916-09) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 57 kD
Application
Fig3: Blank control:Molt-4. Primary Antibody (green line): Rabbit Anti-ROR Gamma antibody (ER1916-09) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Application
Fig4: Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (RORG) Polyclonal Antibody, Unconjugated (ER1916-09) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | Isoform 1: widely expressed in many tissues, including liver and adipose, and highly expressed in skeletal muscle. Isoform 2: Primarily expressed in immature thymocytes. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:1ug/test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
