Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (rat colon tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (RPS6KB1) Polyclonal Antibody, Unconjugated (ER1916-13) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: MCF-7(Human) Cell Lysate at 30 ug U-2OS(Human) Cell Lysate at 30 ug MOLT-4(Human) Cell Lysate at 30 ug Primary: Anti- RPS6KB1 (ER1916-13) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 70 kD Observed band size: 68 kD| Product Name | RPS6KB1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | p70(S6K)-alpha ribosomal protein S6 kinase p70 S6 Kinase P70 Beta1 KS6B1_HUMAN Ribosomal protein S6 kinase beta-1 70 kDa ribosomal protein S6 kinase 1 P70S6K1 p70-S6K 1 Ribosomal protein S6 kinase I Serine/threonine-protein kinase 14A p70 ribosomal S6 kinase alpha p70 S6 kinase alpha p70 S6K-alpha p70 S6KA S6K PS6K S6K1 STK14A p70-S6K p70 S6KA p70-alpha S6K-beta-1 p70(S6K)-alpha. |
| Product description | Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the preinitiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex. The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at 'Thr-412', which is proposed to be a negative feedback mechanism for the RPS6KB1 anti-apoptotic function. |
| Immunogen | KLH conjugated synthetic peptide derived from human p70 S6 kinase alpha 251-350/525 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | p70(S6K)-alpha ribosomal protein S6 kinase p70 S6 Kinase P70 Beta1 KS6B1_HUMAN Ribosomal protein S6 kinase beta-1 70 kDa ribosomal protein S6 kinase 1 P70S6K1 p70-S6K 1 Ribosomal protein S6 kinase I Serine/threonine-protein kinase 14A p70 ribosomal S6 kinase alpha p70 S6 kinase alpha p70 S6K-alpha p70 S6KA S6K PS6K S6K1 STK14A p70-S6K p70 S6KA p70-alpha S6K-beta-1 p70(S6K)-alpha. |
|---|---|
| Molecular Weight(MW) | 70 KDa |
| Cellular Localization | Cell junction, synapse, synaptosome. Mitochondrion outer membrane. |
| SwissProt ID | P23443 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (rat colon tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (RPS6KB1) Polyclonal Antibody, Unconjugated (ER1916-13) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: MCF-7(Human) Cell Lysate at 30 ug U-2OS(Human) Cell Lysate at 30 ug MOLT-4(Human) Cell Lysate at 30 ug Primary: Anti- RPS6KB1 (ER1916-13) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 70 kD Observed band size: 68 kD| Positive Control | Widely expressed. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
