Application
Fig1: Sample: Lung (Mouse) Lysate at 40 ug A431(Human) Cell Lysate at 30 ug Testis (Mouse) Lysate at 40 ug Primary: Anti-RSPH4A (ER1916-15) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 81 kD Observed band size: 81 kDApplication
Fig2: Sample: Trachea (Mouse) Lysate at 40 ug Primary: Anti-RSPH4A (ER1916-15) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 81 kD Observed band size: 81 kD| Product Name | RSPH4A |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CILD11 dJ412I7.1 Radial spoke head protein 4 homolog A Radial spoke head-like protein 3 RSH4A_HUMAN RSHL3 Rsph4a RSPH6B A230081C05. |
| Product description | Probable component of the axonemal radial spoke head. Radial spokes are regularly spaced along cilia, sperm and flagella axonemes. They consist of a thin stalk which is attached to a subfiber of the outer doublet microtubule, and a bulbous head which is attached to the stalk and appears to interact with the projections from the central pair of microtubules. |
| Immunogen | KLH conjugated synthetic peptide derived from human RSPH4A/RSHL3 435-482/716 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | CILD11 dJ412I7.1 Radial spoke head protein 4 homolog A Radial spoke head-like protein 3 RSH4A_HUMAN RSHL3 Rsph4a RSPH6B A230081C05. |
|---|---|
| Molecular Weight(MW) | 81 KDa |
| Cellular Localization | Cytoplasm; cytoskeleton; cilium axoneme. Radial spoke. |
| SwissProt ID | Q5TD94 |
|---|
Application
Fig1: Sample: Lung (Mouse) Lysate at 40 ug A431(Human) Cell Lysate at 30 ug Testis (Mouse) Lysate at 40 ug Primary: Anti-RSPH4A (ER1916-15) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 81 kD Observed band size: 81 kD
Application
Fig2: Sample: Trachea (Mouse) Lysate at 40 ug Primary: Anti-RSPH4A (ER1916-15) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 81 kD Observed band size: 81 kD| Positive Control | Defects in RSPH4A are the cause of primary ciliary dyskinesia type 11 (CILD11) [MIM:612649]. CILD is an autosomal recessive disorder characterized by axonemal abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia; reduced fertility is often observed in male patients due to abnormalities of sperm tails. Half of the patients exhibit situs inversus, due to dysfunction of monocilia at the embryonic node and randomization of left-right body asymmetry. Primary ciliary dyskinesia associated with situs inversus is referred to as Kartagener syndrome. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
