Application
Fig1: Sample: Gourd leaf Lysate at 40 ug Lotus leaf Lysate at 40 ug Primary: Anti-RUBISCO (ER1916-16)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52kD Observed band size: 52 kDApplication
Fig2: Sample: Linden Lysate at 40 ug Primary: Anti-RUBISCO (ER1916-16)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52kD Observed band size: 52 kD| Product Name | RuBisCO large subunit |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | rbcL RBL_SPIOL Ribulose bisphosphate carboxylase large chain RuBisCO-large subunit ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (chloroplast) [Arabidopsis lyrata]. |
| Product description | Ribulose-1,5-bisphosphate carboxylase/oxygenase, commonly known by the abbreviations Rubisco, rubisco, RuBPCase, or RuBPco, is an enzyme involved in the first major step of carbon fixation, a process by which atmospheric carbon dioxide is converted by plants and other photosynthetic organisms to energy-rich molecules such as glucose. In chemical terms, it catalyzes the carboxylation of ribulose-1,5-bisphosphate (also known as RuBP). It is probably the most abundant enzyme on Earth. RuBisCO is important biologically because it catalyzes the primary chemical reaction by which inorganic carbon enters the biosphere. While many autotrophic bacteria and archaea fix carbon via the reductive acetyl CoA pathway, the 3-hydroxypropionate cycle, or the reverse Krebs cycle, these pathways are relatively small contributors to global carbon fixation than that catalyzed by RuBisCO. Phosphoenolpyruvate carboxylase, unlike RuBisCO, only temporarily fixes carbon. Reflecting its importance, RuBisCO is the most abundant protein in leaves, accounting for 50% of soluble leaf protein in C3 plants (20–30% of total leaf nitrogen) and 30% of soluble leaf protein in C4 plants (5–9% of total leaf nitrogen). |
| Immunogen | KLH conjugated synthetic peptide derived from Arabidopsis thaliana Rubisco 151-250/475 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1000: |
|
| Species Reactivity | Arabidopsis Thaliana |
| Concentration | 1mg/ml |
| Alternative Names | rbcL
RBL_SPIOL
Ribulose bisphosphate carboxylase large chain RuBisCO-large subunit ribulose-1 5-bisphosphate carboxylase/oxygenase large subunit ribulose-1 5-bisphosphate carboxylase/oxygenase large subunit (chloroplast) [Arabidopsis lyrata]. |
|---|---|
| Molecular Weight(MW) | 52 KDa |
| Cellular Localization | Plastid, chloroplast. |
| SwissProt ID | P00875 |
|---|
Application
Fig1: Sample: Gourd leaf Lysate at 40 ug Lotus leaf Lysate at 40 ug Primary: Anti-RUBISCO (ER1916-16)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52kD Observed band size: 52 kD
Application
Fig2: Sample: Linden Lysate at 40 ug Primary: Anti-RUBISCO (ER1916-16)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52kD Observed band size: 52 kD| Positive Control | Gourd leaf Lysate, Linden Lysate. |
|---|---|
| Application Notes | WB:1:500-1000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
