Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (RUSC1) Polyclonal Antibody, Unconjugated (ER1916-17) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample:Hela (Human)Cell Lysate at 40 ug Primary: Anti-RUSC1(ER1916-17)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 96kD Observed band size: 105kD| Product Name | RUSC1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DKFZp761A1822 Nesca New molecule containing SH3 at the carboxy terminus RUN and SH3 domain containing 1 RUN and SH3 domain containing protein 1 RUSC 1. |
| Product description | RUSC1 is a putative signaling adapter which may play a role in neuronal differentiation and may be involved in regulation of NGF-dependent neurite outgrowth. It seems to be involved in signaling pathways that are regulated by the prolonged activation of MAPK. |
| Immunogen | KLH conjugated synthetic peptide derived from human RUSC1 281-380/902 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatPigCowSheep |
| Concentration | 1mg/ml |
| Alternative Names | DKFZp761A1822 Nesca New molecule containing SH3 at the carboxy terminus RUN and SH3 domain containing 1 RUN and SH3 domain containing protein 1 RUSC 1. |
|---|---|
| Molecular Weight(MW) | 96 KDa |
| Cellular Localization | Cytoplasm. Nucleus. Note=Translocated to the nuclear envelope upon stimulation with NGF. |
| SwissProt ID | Q9BVN2 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (RUSC1) Polyclonal Antibody, Unconjugated (ER1916-17) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample:Hela (Human)Cell Lysate at 40 ug Primary: Anti-RUSC1(ER1916-17)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 96kD Observed band size: 105kD| Positive Control | Predominantly expressed in brain. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
