Application
Fig1: Sample: Heart(Mouse) Cell Lysate at 40 ug Primary: Anti-SAMD8 (ER1916-19) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 48 kD| Product Name | SAMD8 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 1110053F04Rik 1700010P07Rik FLJ25082 MGC118303 RP11-487I5.5 SMSr Sphingomyelin synthase related Sphingomyelin synthase related protein 1 Sterile alpha motif domain containing 8. |
| Product description | Sphingomyelin synthases synthesize sphingolipids through transfer of a phosphatidyl head group on to the primary hydroxyl of ceramide. SAMD8 is an endoplasmic reticulum (ER) transferase that has no sphingomyelin synthase activity but can convert phosphatidylethanolamine (PE) and ceramide to ceramide phosphoethanolamine (CPE) albeit with low product yield. Appears to operate as a ceramide sensor to control ceramide homeostasis in the endoplasmic reticulum rather than a converter of ceramides. Seems to be critical for the integrity of the early secretory pathway. |
| Immunogen | KLH conjugated synthetic peptide derived from human SAMD8 81-180/415 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | 1110053F04Rik 1700010P07Rik FLJ25082 MGC118303 RP11-487I5.5 SMSr Sphingomyelin synthase related Sphingomyelin synthase related protein 1 Sterile alpha motif domain containing 8. |
|---|---|
| Molecular Weight(MW) | 48 KDa |
| SwissProt ID | Q96LT4 |
|---|
Application
Fig1: Sample: Heart(Mouse) Cell Lysate at 40 ug Primary: Anti-SAMD8 (ER1916-19) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 48 kD| Application Notes | WB:1:500-2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
