Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SCD1) Polyclonal Antibody, Unconjugated (ER1916-26) at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SCD1) Polyclonal Antibody, Unconjugated (ER1916-26) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SCD1) Polyclonal Antibody, Unconjugated (ER1916-26) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig4: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-SCD1 Polyclonal Antibody, Unconjugated(ER1916-26) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig5: Sample:Lung (Rat) Lysate at 40 ug Primary: Anti- SCD1 (ER1916-26) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 41 kD Observed band size: 55 kD| Product Name | SCD1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACOD_HUMAN Acyl-CoA desaturase Delta(9)-desaturase Delta-9 desaturase Delta-9-Desaturase FADS5 Fatty acid desaturase PRO0998 SCD 1 SCD SCD1 Stearoyl-CoA Desaturase. |
| Product description | Terminal component of the liver microsomal stearyl-CoA desaturase system, that utilizes O(2) and electrons from reduced cytochrome b5 to catalyze the insertion of a double bond into a spectrum of fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA. |
| Immunogen | KLH conjugated synthetic peptide derived from human SCD1 265-359/359 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | ACOD_HUMAN Acyl-CoA desaturase Delta(9)-desaturase Delta-9 desaturase Delta-9-Desaturase FADS5 Fatty acid desaturase PRO0998 SCD 1 SCD SCD1 Stearoyl-CoA Desaturase. |
|---|---|
| Molecular Weight(MW) | 41 KDa |
| Cellular Localization | Endoplasmic reticulum membrane; Multi-pass membrane protein (Probable). |
| SwissProt ID | O00767 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SCD1) Polyclonal Antibody, Unconjugated (ER1916-26) at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SCD1) Polyclonal Antibody, Unconjugated (ER1916-26) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SCD1) Polyclonal Antibody, Unconjugated (ER1916-26) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig4: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-SCD1 Polyclonal Antibody, Unconjugated(ER1916-26) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig5: Sample:Lung (Rat) Lysate at 40 ug Primary: Anti- SCD1 (ER1916-26) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 41 kD Observed band size: 55 kD| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
