Application
Fig1: Sample: Cartilage(Rat) Lysate at 40 ug Primary: Anti-Sclerostin (ER1916-29) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 21 kD| Product Name | Sclerostin |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BEER Cortical hyperostosis with syndactyly Sclerosteosis Sclerostin SOST SOST_HUMAN VBCH. |
| Product description | Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation. |
| Immunogen | KLH conjugated synthetic peptide derived from human Sclerostin 121-180/213 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | BEER Cortical hyperostosis with syndactyly Sclerosteosis Sclerostin SOST SOST_HUMAN VBCH. |
|---|---|
| Molecular Weight(MW) | 21 KDa |
| Cellular Localization | Secreted. |
| SwissProt ID | Q9BQB4 |
|---|
Application
Fig1: Sample: Cartilage(Rat) Lysate at 40 ug Primary: Anti-Sclerostin (ER1916-29) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 21 kD| Positive Control | Widely expressed at low levels with highest levels in bone, cartilage, kidney, liver, bone marrow and primary osteeoblasts differentiated for 21 days. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
