Application
Fig1: Protein: spleen(mouse) lysate at 40ug; Primary: rabbit Anti-Secretogranin V (ER1916-33) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size:17/21 kD Observed band size:21 kDApplication
Fig2: Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti-Secretogranin V(ER1916-33) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17/21 kD Observed band size: 21 kD| Product Name | Secretogranin V |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 7B2 7B2 protein APPG Neuroendocrine protein 7B2 P7B2 Pituitary polypeptide Pituitary polypeptide Prohormone convertase chaperone SCG 5 SCG5 Secretogranin 5 Secretogranin V 7B2 protein Secretogranin5 SecretograninV Secretory granule endocrine protein I Secretory granule endocrine protein I Secretory granule neuroendocrine protein 1 7B2 protein Secretory granule neuroendocrine protein 1 Sg V SGNE 1 SGNE1 SgV 7B2_HUMAN. |
| Product description | Secretogranin V may have intracellular functions other than PC2 maturation in certain cells, although some recent work shows a discordancy between mRNA expression and enzymic activity. The mechanism for PC2 maturation appears to involve pro-Secretogranin V binding to inactive pro-PC2 via its polyproline motif (116 PPNPCP 121) in the endoplasmic reticulum and chaperoning pro-PC2 to a later secretory pathway compartment where maturation of PC2 proceeds once pro-Secretogranin V is proteolytically processed by a furin-like convertase into a 21kDa fragment and a C-terminal peptide. It is the C-terminal peptide that potently inhibits maturation of pro-PC2 until the complex is subjected to a decreasing pH gradient along the secretory pathway. It seems likely that mature PC2 then cleaves the CT peptide at its internal lys-lys site. Secretogranin V knockout mice develop a severe Cushings-like phenotype and exhibit multiple metabolic and hormonal abnormalities, indicating that Secretogranin V is required for activation of PC2 in vivo. Conversely, PC2-null mice appear to be viable. |
| Immunogen | KLH conjugated synthetic peptide derived from human PHF21A 1-100/212 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | 7B2 7B2 protein APPG Neuroendocrine protein 7B2 P7B2 Pituitary polypeptide Pituitary polypeptide Prohormone convertase chaperone SCG 5 SCG5 Secretogranin 5 Secretogranin V 7B2 protein Secretogranin5 SecretograninV Secretory granule endocrine protein I Secretory granule endocrine protein I Secretory granule neuroendocrine protein 1 7B2 protein Secretory granule neuroendocrine protein 1 Sg V SGNE 1 SGNE1 SgV 7B2_HUMAN. |
|---|---|
| Molecular Weight(MW) | 17/21 KDa |
| Cellular Localization | Secreted. Note=Neuroendocrine and endocrine secretory granules. |
| SwissProt ID | P05408 |
|---|
Application
Fig1: Protein: spleen(mouse) lysate at 40ug; Primary: rabbit Anti-Secretogranin V (ER1916-33) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size:17/21 kD Observed band size:21 kD
Application
Fig2: Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti-Secretogranin V(ER1916-33) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17/21 kD Observed band size: 21 kD| Positive Control | Highly expressed in brain. Expressed at much lower level in other tissues. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
