Application
Fig1: Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti-Sema3A (ER1916-34) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 86 kD Observed band size: 86 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Sema3A) Polyclonal Antibody, Unconjugated (ER1916-34) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.| Product Name | Sema3A |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Semaphorin 3A Semaphorin-3A Semaphorin-3A precursor Semaphorin3A SemD Sema 3A SEM3A_MOUSE Coll 1 Coll1 Hsema I Hsema III sema domain immunoglobulin domain (Ig) short basic domain secreted (semaphorin) 3A Sema III SEMA1 SEMA1-PEN SEMA3A SEMAD SEMAIII SEMAL Semaphorin III Semaphorin like growth cone guidance homolog of chick collapsin Semaphorin III Semaphorin-D Sema D. |
| Product description | Plays a role in growth cones guidance. May function to pattern sensory projections by selectively repelling axons that normally terminate dorsally. Involved in the development of the olfactory system and in neuronal control of puberty. |
| Immunogen | KLH conjugated synthetic peptide derived from mouse Sema3A 22-120/772 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Semaphorin 3A Semaphorin-3A Semaphorin-3A precursor Semaphorin3A SemD Sema 3A SEM3A_MOUSE Coll 1 Coll1 Hsema I Hsema III sema domain immunoglobulin domain (Ig) short basic domain secreted (semaphorin) 3A Sema III SEMA1 SEMA1-PEN SEMA3A SEMAD SEMAIII SEMAL Semaphorin III Semaphorin like growth cone guidance homolog of chick collapsin Semaphorin III Semaphorin-D Sema D. |
|---|---|
| Molecular Weight(MW) | 86 KDa |
| Cellular Localization | Secreted. |
| SwissProt ID | O08665 |
|---|
Application
Fig1: Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti-Sema3A (ER1916-34) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 86 kD Observed band size: 86 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Sema3A) Polyclonal Antibody, Unconjugated (ER1916-34) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.| Positive Control | Expressed at relatively high levels in brain and muscle, moderate levels in lung, bursa, and heart and virtually absent in liver. Collapsin-1, -2, -3, and -5 bind to overlapping but distinct axon tracts. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
