Application
Fig1: Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti-SEMA4A (ER1916-35) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 80 kD Observed band size: 83 kDApplication
Fig2: Sample: HepG2(Human) Cell Lysate at 30 ug Primary: Anti-SEMA4A (ER1916-35) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 80 kD Observed band size: 83 kDApplication
Fig3: Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SEMA4A) Polyclonal Antibody, Unconjugated (ER1916-35) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | SEMA4A |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CORD10 RP11 54H19 2 RP35 Sema B Sema domain immunoglobulin domain Ig transmembrane domain TM and short cytoplasmic domain 4A Sema domain immunoglobulin domain Ig transmembrane domain TM and short cytoplasmic domain semaphorin 4A SEMAB Semaphorin 4A precursor Semaphorin B SEMB. |
| Product description | Cell surface receptor for PLXNB1, PLXNB2, PLXNB3 and PLXND1 that plays an important role in cell-cell signaling. Plays a role in priming antigen-specific T cells, promotes differentiation of Th1 T helper cells, and thereby contributes to adaptive immunity. Promotes phosphorylation of TIMD2. Inhibits angiogenesis. Promotes axon growth cone collapse. Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons (By similarity). |
| Immunogen | KLH conjugated synthetic peptide derived from human SEMA4A 361-460/761 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowRabbit |
| Concentration | 1mg/ml |
| Alternative Names | CORD10 RP11 54H19 2 RP35 Sema B Sema domain immunoglobulin domain Ig transmembrane domain TM and short cytoplasmic domain 4A Sema domain immunoglobulin domain Ig transmembrane domain TM and short cytoplasmic domain semaphorin 4A SEMAB Semaphorin 4A precursor Semaphorin B SEMB. |
|---|---|
| Molecular Weight(MW) | 80 KDa |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. |
| SwissProt ID | Q9H3S1 |
|---|
Application
Fig1: Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti-SEMA4A (ER1916-35) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 80 kD Observed band size: 83 kD
Application
Fig2: Sample: HepG2(Human) Cell Lysate at 30 ug Primary: Anti-SEMA4A (ER1916-35) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 80 kD Observed band size: 83 kD
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SEMA4A) Polyclonal Antibody, Unconjugated (ER1916-35) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
