Application
Fig1: Blank control: Raji(blue). Primary Antibody:Rabbit Anti-SEMA4D antibody(ER1916-36), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody (ER1916-36, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.Application
Fig2: Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti-SEMA4D (ER1916-36) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60/126 kD Observed band size: 60 kD| Product Name | SEMA4D |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | A8 antibody BB 18 BB18 CD 100 CD100 CD100 antigen Coll 4 COLL4 Collapsin 4 Collapsin4 GR3 Human semaphorin CD100 mRNA complete cds Leukocyte activation antigen CD100 M sema G MSEMA SEM4D_HUMAN Sema 4d Sema domain immunoglobulin domain Ig transmembrane domain TM and short cytoplasmic domain semaphorin 4D Sema H SEMA J SEMA4D Semacl 2 Semacl2 SemaH SEMAJ Semaphorin 4D Semaphorin 4D precursor Semaphorin C like 2 Semaphorin H Semaphorin J Semaphorin-4D Semaphorin4D SemaphorinJ Semcl 2 Semcl2. |
| Immunogen | KLH conjugated synthetic peptide derived from human SEMA4D 75-170/862 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg /Test: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | A8 antibody BB 18 BB18 CD 100 CD100 CD100 antigen Coll 4 COLL4 Collapsin 4 Collapsin4 GR3 Human semaphorin CD100 mRNA complete cds Leukocyte activation antigen CD100 M sema G MSEMA SEM4D_HUMAN Sema 4d Sema domain immunoglobulin domain Ig transmembrane domain TM and short cytoplasmic domain semaphorin 4D Sema H SEMA J SEMA4D Semacl 2 Semacl2 SemaH SEMAJ Semaphorin 4D Semaphorin 4D precursor Semaphorin C like 2 Semaphorin H Semaphorin J Semaphorin-4D Semaphorin4D SemaphorinJ Semcl 2 Semcl2. |
|---|---|
| Molecular Weight(MW) | 60, 126 KDa |
| Cellular Localization | Membrane. |
| SwissProt ID | Q92854 |
|---|
Application
Fig1: Blank control: Raji(blue). Primary Antibody:Rabbit Anti-SEMA4D antibody(ER1916-36), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody (ER1916-36, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
Application
Fig2: Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti-SEMA4D (ER1916-36) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60/126 kD Observed band size: 60 kD| Positive Control | Strongly expressed in skeletal muscle, peripheral blood lymphocytes, spleen, and thymus and also expressed at lower levels in testes, brain, kidney, small intestine, prostate, heart, placenta, lung and pancreas, but not in colon and liver. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1μg /Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
