Application
Fig1: Sample: DU145(Human) Cell Lysate at 30 ug Primary: Anti- Semaphorin 3E (ER1916-37) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 86 kD Observed band size: 111 kDApplication
Fig2: Sample: Lymph node (Mouse) Lysate at 40 ug Primary: Anti- Semaphorin 3E (ER1916-37) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 86 kD Observed band size: 111 kD| Product Name | Semaphorin 3E |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Coll 5 Coll5 KIAA0331 M sema H M SEMAH M SemaK Msema H MSEMAH MSemaK SEM3E_HUMAN Sema domain immunoglobulin domain (Ig) Sema domain immunoglobulin domain (Ig) short basic domain secreted 3E Sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E SEMA3E SEMAH Semaphorin 3E Semaphorin-3E Semaphorin3E Short basic domain secreted (semaphorin) 3E SEM3E_HUMAN. |
| Product description | Plays an important role in signaling via the cell surface receptor PLXND1. Mediates reorganization of the actin cytoskeleton, leading to the retraction of cell projections. Promotes focal adhesion disassembly and inhibits adhesion of endothelial cells to the extracellular matrix. Regulates angiogenesis, both during embryogenesis and after birth. Can down-regulate sprouting angiogenesis. Required for normal vascular patterning during embryogenesis. Plays an important role in ensuring the specificity of synapse formation |
| Immunogen | KLH conjugated synthetic peptide derived from human SEMA3E/Semaphorin 3E 401-450/775 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | Coll 5
Coll5
KIAA0331
M sema H
M SEMAH
M SemaK
Msema H
MSEMAH
MSemaK
SEM3E_HUMAN
Sema domain immunoglobulin domain (Ig)
Sema domain immunoglobulin domain (Ig) short basic domain secreted 3E
Sema domain immunoglobulin domain (Ig) short basic domain secreted (semaphorin) 3E SEMA3E SEMAH Semaphorin 3E Semaphorin-3E Semaphorin3E Short basic domain secreted (semaphorin) 3E SEM3E_HUMAN. |
|---|---|
| Molecular Weight(MW) | 86 KDa |
| Cellular Localization | Secreted. |
| SwissProt ID | O15041 |
|---|
Application
Fig1: Sample: DU145(Human) Cell Lysate at 30 ug Primary: Anti- Semaphorin 3E (ER1916-37) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 86 kD Observed band size: 111 kD
Application
Fig2: Sample: Lymph node (Mouse) Lysate at 40 ug Primary: Anti- Semaphorin 3E (ER1916-37) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 86 kD Observed band size: 111 kD| Application Notes | WB:1:500-2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
