Application
Fig1: Sample: A549(Human) Cell Lysate at 30 ug Primary: Anti-Semaphorin 5A (ER1916-38) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 118 kD Observed band size: 118 kD| Product Name | Semaphorin 5A |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Semaphorin 5A Semaphorin-5A Semaphorin5A SEMA5A SEMAF Semaphorin F SEM5A_HUMAN. |
| Product description | Bifunctional axonal guidance cue regulated by sulfated proteoglycans; attractive effects result from interactions with heparan sulfate proteoglycans (HSPGs), while the inhibitory effects depend on interactions with chondroitin sulfate proteoglycans (CSPGs) (By similarity). Ligand for receptor PLXNB3. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1. May promote angiogenesis by increasing endothelial cell proliferation and migration and inhibiting apoptosis. |
| Immunogen | KLH conjugated synthetic peptide derived from human Semaphorin 5A 671-770/1074 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | Semaphorin 5A Semaphorin-5A Semaphorin5A SEMA5A SEMAF Semaphorin F SEM5A_HUMAN. |
|---|---|
| Molecular Weight(MW) | 118 KDa |
| Cellular Localization | Membrane; single-pass type I membrane protein |
| SwissProt ID | Q13591 |
|---|
Application
Fig1: Sample: A549(Human) Cell Lysate at 30 ug Primary: Anti-Semaphorin 5A (ER1916-38) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 118 kD Observed band size: 118 kD| Application Notes | WB:1:500-2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 |
