Fig1: Protein: bone(mouse) lysate at 40ug;
Primary: rabbit Anti-SEPHS1(ER1916-40) at 1:300;
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 36 kD
Positive Control
Isoform 1 and isoform 2 are gradually expressed during the cell cycle until G2/M phase and then decreased. Isoform 3 is gradually expressed during the cell cycle until S phase and then decreased.
Application Notes
WB:1:500-2000:
Additional Information
Form
Liquid
Storage Instructions
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
Storage Buffer
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.