Application
Fig1: Sample: Jurkat Cell (Human) Lysate at 40 ug RajiCell (Human) Lysate at 40 ug K562 Cell (Human) Lysate at 40 ug Primary: Anti-SERCA3(ER1916-41)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 114kD Observed band size: 114kD| Product Name | SERCA3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Adenosine triphosphatase calcium AT2A3_HUMAN Atp2a3 ATPase Ca(2+) transporting ubiquitous ATPase Ca++ transporting ubiquitous Calcium pump 3 Calcium translocating P type ATPase Sarco/endoplasmic reticulum Ca2+ ATPase Sarcoplasmic/endoplasmic reticulum calcium ATPase 3 SERCA 3 SERCA3 SERCA3b SR Ca(2+) ATPase 3 SR Ca(2+)-ATPase 3. |
| Product description | This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Transports calcium ions from the cytosol into the sarcoplasmic/endoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction. |
| Immunogen | KLH conjugated synthetic peptide derived from human SERCA3 101-200/1043 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseChickenCowXenopus |
| Concentration | 1mg/ml |
| Alternative Names | Adenosine triphosphatase calcium AT2A3_HUMAN Atp2a3 ATPase Ca(2+) transporting ubiquitous ATPase Ca++ transporting ubiquitous Calcium pump 3 Calcium translocating P type ATPase Sarco/endoplasmic reticulum Ca2+ ATPase Sarcoplasmic/endoplasmic reticulum calcium ATPase 3 SERCA 3 SERCA3 SERCA3b SR Ca(2+) ATPase 3 SR Ca(2+)-ATPase 3. |
|---|---|
| Molecular Weight(MW) | 114 KDa |
| Cellular Localization | Nucleus membrane. Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. |
| SwissProt ID | Q93084 |
|---|
Application
Fig1: Sample: Jurkat Cell (Human) Lysate at 40 ug RajiCell (Human) Lysate at 40 ug K562 Cell (Human) Lysate at 40 ug Primary: Anti-SERCA3(ER1916-41)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 114kD Observed band size: 114kD| Positive Control | Found in most tissues. Most abundant in thymus, trachea, salivary gland, spleen, bone marrow, lymph node, peripheral leukocytes, pancreas and colon. Also detected in fetal tissues. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
