Application
Fig1: Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti- Serine racemase (ER1916-43) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD Observed band size: 37 kDApplication
Fig2: Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation:Anti-Serine racemase Polyclonal Antibody, Unconjugated(ER1916-43) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig3: Sample: lovo (human)cell Lysate at 40 ug Primary: Anti- Serine racemase (ER1916-43) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD Observed band size: 37 kD| Product Name | Serine racemase |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | D serine ammonia lyase D serine dehydratase D-serine ammonia-lyase D-serine dehydratase ILV1 ISO1 L serine ammonia lyase L serine dehydratase L-serine ammonia-lyase L-serine dehydratase Serine racemase srr SRR_HUMAN. |
| Product description | Catalyzes the synthesis of D-serine from L-serine. D-serine is a key coagonist with glutamate at NMDA receptors. Has dehydratase activity towards both L-serine and D-serine. |
| Immunogen | KLH conjugated synthetic peptide derived from human Serine racemase 1-100/430 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogCowRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | D serine ammonia lyase D serine dehydratase D-serine ammonia-lyase D-serine dehydratase ILV1 ISO1 L serine ammonia lyase L serine dehydratase L-serine ammonia-lyase L-serine dehydratase Serine racemase srr SRR_HUMAN. |
|---|---|
| Molecular Weight(MW) | 37 KDa |
| SwissProt ID | Q9GZT4 |
|---|
Application
Fig1: Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti- Serine racemase (ER1916-43) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD Observed band size: 37 kD
Application
Fig2: Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation:Anti-Serine racemase Polyclonal Antibody, Unconjugated(ER1916-43) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig3: Sample: lovo (human)cell Lysate at 40 ug Primary: Anti- Serine racemase (ER1916-43) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD Observed band size: 37 kD| Positive Control | Brain: expressed at high levels in hippocampus and corpus callosum, intermediate levels in substantia nigra and caudate, and low levels in amygdala, thalamus, and subthalamic nuclei. Expressed in heart, skeletal muscle, kidney and liver. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
