Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-SLC6A4 (ER1916-44) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 70 kD Observed band size: 70 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Serotonin transporter) Polyclonal Antibody, Unconjugated (ER1916-44) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Serotonin transporter) Polyclonal Antibody, Unconjugated (ER1916-44) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.| Product Name | Serotonin transporter |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 5HT transporter 5-HTT 5-HTTLPR 5HTT hSERT HTT OCD1 SERT SERT1 SC6A4_HUMAN SERT SLC6A4 Sodium dependent serotonin transporter Sodium-dependent serotonin transporter Solute carrier family 6 member 4 sodium-dependent serotonin transporter 5-hydroxytryptamine transporter 5HT transporter Na+/Cl- dependent serotonin transporter. |
| Product description | Serotonin transporter whose primary function in the central nervous system involves the regulation of serotonergic signaling via transport of serotonin molecules from the synaptic cleft back into the pre-synaptic terminal for re-utilization. Plays a key role in mediating regulation of the availability of serotonin to other receptors of serotonergic systems. Terminates the action of serotonin and recycles it in a sodium-dependent manner. |
| Immunogen | KLH conjugated synthetic peptide derived from human 5-HTT 401-500/630 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowSheepGuinea Pig |
| Concentration | 1mg/ml |
| Alternative Names | 5HT transporter 5-HTT 5-HTTLPR 5HTT hSERT HTT OCD1 SERT SERT1 SC6A4_HUMAN SERT SLC6A4 Sodium dependent serotonin transporter Sodium-dependent serotonin transporter Solute carrier family 6 member 4 sodium-dependent serotonin transporter 5-hydroxytryptamine transporter 5HT transporter Na+/Cl- dependent serotonin transporter. |
|---|---|
| Molecular Weight(MW) | 70 KDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. Endomembrane system; Multi-pass membrane protein. Endosome membrane; Multi-pass membrane protein. |
| SwissProt ID | P31645 |
|---|
Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-SLC6A4 (ER1916-44) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 70 kD Observed band size: 70 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Serotonin transporter) Polyclonal Antibody, Unconjugated (ER1916-44) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Serotonin transporter) Polyclonal Antibody, Unconjugated (ER1916-44) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.| Positive Control | Expressed in platelets (at protein level). |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
