Application
Fig1: Sample: K562(Human) Cell Lysate at 30 ug Primary: Anti- SIAH2 (ER1916-58) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 36 kD| Product Name | SIAH2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Seven in Absentia Homolog 2 Ubiquitin Ligase Siah2 SIAH2_HUMAN E3 ubiquitin-protein ligase SIAH2 Seven in absentia homolog 2 Siah-2 hSiah2. |
| Product description | E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes. Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (POU2AF1, PML, NCOR1), a cell surface receptor (DCC), an antiapoptotic protein (BAG1), and a protein involved in synaptic vesicle function in neurons (SYP). Mediates ubiquitination and proteasomal degradation of DYRK2 in response to hypoxia. It is thereby involved in apoptosis, tumor suppression, cell cycle, transcription and signaling processes. Has some overlapping function with SIAH1. Triggers the ubiquitin-mediated degradation of TRAF2, whereas SIAH1 can not. Promotes monoubiquitination of SNCA. |
| Immunogen | KLH conjugated synthetic peptide derived from human SIAH2 201-300/324 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Seven in Absentia Homolog 2 Ubiquitin Ligase Siah2 SIAH2_HUMAN E3 ubiquitin-protein ligase SIAH2 Seven in absentia homolog 2 Siah-2 hSiah2. |
|---|---|
| Molecular Weight(MW) | 35 KDa |
| Cellular Localization | Cytoplasm. Nucleus. |
| SwissProt ID | O43255 |
|---|
Application
Fig1: Sample: K562(Human) Cell Lysate at 30 ug Primary: Anti- SIAH2 (ER1916-58) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 36 kD| Positive Control | Widely expressed at low level. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
