Application
Fig1: Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti- SIGLEC10 (ER1916-59) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 77 kD Observed band size: 102 kD| Product Name | SIGLEC10 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRO940 mSiglec G Sialic acid binding Ig like lectin 10 Sialic acid binding Ig like lectin 10 sialic acid binding Ig like lectin 10 Ig like lectin 7 sialic acid binding Ig like lectin G Sialic acid binding Immunoglobulin like lectin 10 SIGLEC 10 Siglec G siglec like gene 2 Siglec like protein 2 Siglec like protein 2 Siglecg SLG2. |
| Product description | Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- or alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. |
| Immunogen | KLH conjugated synthetic peptide derived from human SIGLEC10 321-420/697 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogPig |
| Concentration | 1mg/ml |
| Alternative Names | PRO940 mSiglec G Sialic acid binding Ig like lectin 10 Sialic acid binding Ig like lectin 10 sialic acid binding Ig like lectin 10 Ig like lectin 7 sialic acid binding Ig like lectin G Sialic acid binding Immunoglobulin like lectin 10 SIGLEC 10 Siglec G siglec like gene 2 Siglec like protein 2 Siglec like protein 2 Siglecg SLG2. |
|---|---|
| Molecular Weight(MW) | 77 KDa |
| Cellular Localization | Isoform 1, 2, 3, 4: Cell membrane; Single-pass type I membrane protein. Isoform 5: Secreted. |
| SwissProt ID | Q96LC7 |
|---|
Application
Fig1: Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti- SIGLEC10 (ER1916-59) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 77 kD Observed band size: 102 kD| Positive Control | Expressed by peripheral blood leukocytes (eosinophils, monocytes and a natural killer cell subpopulation). Isoform 5 is found to be the most abundant isoform. Found in lymph node, lung, ovary and appendix. Isoform 1 is found at high levels and isoform 2 at lower levels in bone marrow, spleen and spinal chord. Isoform 2 is also found in brain. Isoform 4 is specifically found in natural killer cells. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
