Application
Fig1: Sample: 293T(Human) Cell Lysate at 40 ug Primary: Anti-SKP2 (ER1916-62) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 48 kDApplication
Fig2: Blank control: Hela(blue). Primary Antibody:Rabbit Anti- phospho-SKP2 antibody(ER1916-62), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. Antibody (ER1916-62, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of ER1916-62 at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.| Product Name | SKP2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | p45skp2 skp2p45 p45 skp2 p45 S-phase kinase-associated protein 2 SKP2 CDK2/Cyclin A associated protein p45 Cyclin A/CDK2 associated protein p45 F box protein Skp2 F box/LRR repeat protein 1 FBL 1 FBL1 FBXL 1 FBXL1 FLB 1 FLB1 MGC1366 SKP2_HUMAN. |
| Product description | Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, MLL, CDK9, RAG2, FOXO1, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2. Promotes ubiquitination and destruction of CDH1 in a CK1-Dependent Manner, thereby regulating cell migration. |
| Immunogen | KLH conjugated synthetic peptide derived from human Skp2 351-424/424 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:1μg/Test: |
|
| Species Reactivity | HumanMouseRatCowRabbit |
| Concentration | 1mg/ml |
| Alternative Names | p45skp2
skp2p45
p45 skp2 p45 S-phase kinase-associated protein 2 SKP2 CDK2/Cyclin A associated protein p45 Cyclin A/CDK2 associated protein p45 F box protein Skp2 F box/LRR repeat protein 1 FBL 1 FBL1 FBXL 1 FBXL1 FLB 1 FLB1 MGC1366 SKP2_HUMAN. |
|---|---|
| Molecular Weight(MW) | 48 KDa |
| Cellular Localization | Cytoplasm. Nucleus. |
| SwissProt ID | Q13309 |
|---|
Application
Fig1: Sample: 293T(Human) Cell Lysate at 40 ug Primary: Anti-SKP2 (ER1916-62) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 48 kD
Application
Fig2: Blank control: Hela(blue). Primary Antibody:Rabbit Anti- phospho-SKP2 antibody(ER1916-62), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. Antibody (ER1916-62, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of ER1916-62 at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.| Positive Control | 293T Cell Lysate, Hela cell, human thyroid gland carcinoma tissue. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:1μg/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
