Application
Fig1: Sample: K562 (human)cell Lysate at 40 ug Primary: Anti- SLC10A1 (ER1916-66) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38 kD Observed band size: 48 kDApplication
Fig2: Blank control: 293T(blue). Primary Antibody:Rabbit Anti-SLC10A1 antibody(ER1916-66), Dilution: 1μg in 100 1μL 1X PBS containing 0.5% BSA(green); Isotype Control Antibody: Rabbit IgG(orange) used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.| Product Name | SLC10A1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Na+/taurocholate Cotransporting Polypeptide Na(+)/bile acid cotransporter Cell growth-inhibiting gene 29 protein Na / bile acid cotransporter Na / taurocholate transport protein Na(+)/taurocholate transport protein NTCP NTCP1 Sodium/taurocholate cotransporting polypeptide Solute carrier family 10 member 1 Slc10a1 protein Slc10a1 SBACT SLC10A1 NTCP_HUMAN. |
| Product description | Sodium/bile acid cotransporter also known as the Na+-taurocholate cotransporting polypeptide (NTCP) or liver bile acid transporter (LBAT) is a protein that in humans is encoded by the SLC10A1 (solute carrier family 10 member 1) gene. Bile acid:sodium symporters participate in the enterohepatic circulation of bile acids. Two homologous transporters are involved in the reabsorption of bile acids. One of these absorbs bile acids from the intestinal lumen, the bile duct, and the kidney with an apical localization (ileal sodium/bile acid cotransporter). The other is this protein and is expressed in the basolateral membranes of hepatocytes (NTCP). As a cotransporter, NTCP binds two sodium ions and one (conjugated) bile salt molecule, thereby providing a hepatic influx of bile salts. Other transported molecules include steroid hormones, thyroid hormones and various xenobiotics. |
| Immunogen | KLH conjugated synthetic peptide derived from human NTCP 21-120/349 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICC/IFFC |
| WB:1:500-2000: FC:1μg /test: IF:1:100-500: ICC:1:100-500: |
|
| Species Reactivity | HumanMouseRatDogHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Na+/taurocholate Cotransporting Polypeptide
Na(+)/bile acid cotransporter
Cell growth-inhibiting gene 29 protein Na / bile acid cotransporter Na / taurocholate transport protein Na(+)/taurocholate transport protein NTCP NTCP1 Sodium/taurocholate cotransporting polypeptide Solute carrier family 10 member 1 Slc10a1 protein Slc10a1 SBACT SLC10A1 NTCP_HUMAN. |
|---|---|
| Molecular Weight(MW) | 38 KDa |
| Cellular Localization | Membrane; Multi-pass membrane protein. |
| SwissProt ID | Q14973 |
|---|
Application
Fig1: Sample: K562 (human)cell Lysate at 40 ug Primary: Anti- SLC10A1 (ER1916-66) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38 kD Observed band size: 48 kD
Application
Fig2: Blank control: 293T(blue). Primary Antibody:Rabbit Anti-SLC10A1 antibody(ER1916-66), Dilution: 1μg in 100 1μL 1X PBS containing 0.5% BSA(green); Isotype Control Antibody: Rabbit IgG(orange) used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.| Positive Control | Liver and kidney |
|---|---|
| Application Notes | WB:1:500-2000: FC:1μg /test: IF:1:100-500: ICC:1:100-500: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
