Application
Fig1: Sample:Hcclm3 (Human)cell Lysate at 40 ug Primary: Anti-SLC22A1(ER1916-70)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 61kD Observed band size: 61kD| Product Name | SLC22A1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | hOCT1 OCT1 oct1_cds Organic cation transporter 1 S22A1_HUMAN Slc22a1 solute carrier family 22 (organic cation transporter), member 1 Solute carrier family 22 member 1. |
| Product description | Translocates a broad array of organic cations with various structures and molecular weights including the model compounds 1-methyl-4-phenylpyridinium (MPP), tetraethylammonium (TEA), N-1-methylnicotinamide (NMN), 4-(4-(dimethylamino)styryl)-N-methylpyridinium (ASP), the endogenous compounds choline, guanidine, histamine, epinephrine, adrenaline, noradrenaline and dopamine, and the drugs quinine, and metformin. The transport of organic cations is inhibited by a broad array of compounds like tetramethylammonium (TMA), cocaine, lidocaine, NMDA receptor antagonists, atropine, prazosin, cimetidine, TEA and NMN, guanidine, cimetidine, choline, procainamide, quinine, tetrabutylammonium, and tetrapentylammonium. Translocates organic cations in an electrogenic and pH-independent manner. Translocates organic cations across the plasma membrane in both directions. Transports the polyamines spermine and spermidine. Transports pramipexole across the basolateral membrane of the proximal tubular epithelial cells. The choline transport is activated by MMTS. Regulated by various intracellular signaling pathways including inhibition by protein kinase A activation, and endogenously activation by the calmodulin complex, the calmodulin-dependent kinase II and LCK tyrosine kinase. |
| Immunogen | KLH conjugated synthetic peptide derived from human SLC22A1 51-150/554 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | hOCT1
OCT1
oct1_cds
Organic cation transporter 1
S22A1_HUMAN
Slc22a1
solute carrier family 22 (organic cation transporter) member 1 Solute carrier family 22 member 1. |
|---|---|
| Molecular Weight(MW) | 61 KDa |
| Cellular Localization | Basolateral cell membrane. |
| SwissProt ID | O15395 |
|---|
Application
Fig1: Sample:Hcclm3 (Human)cell Lysate at 40 ug Primary: Anti-SLC22A1(ER1916-70)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 61kD Observed band size: 61kD| Positive Control | Widely expressed with high level in liver. Isoform 1 and isoform 2 are expressed in liver. Isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in glial cell lines. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
