Application
Fig1: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-SLC38A2 (ER1916-78) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 56 kDApplication
Fig2: Sample: Intestine (Mouse) Lysate at 40 ug Primary: Anti-SLC38A2 (ER1916-78) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 56 kDApplication
Fig3: Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SLC38A2) Polyclonal Antibody, Unconjugated (ER1916-78) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.| Product Name | SLC38A2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Amino acid transporter 2 Amino acid transporter A2 ATA2 SNAT2 KIAA1382 PRO1068 Protein 40-9-1 S38A2_HUMAN SAT2 Slc38a2 Sodium-coupled neutral amino acid transporter 2 Solute carrier family 38 member 2 System A amino acid transporter System A amino acid transporter 2 System A transporter 1 System N amino acid transporter 2. |
| Product description | Functions as a sodium-dependent amino acid transporter. Mediates the saturable, pH-sensitive and electrogenic cotransport of neutral amino acids and sodium ions with a stoichiometry of 1:1. May function in the transport of amino acids at the blood-brain barrier and in the supply of maternal nutrients to the fetus through the placenta. |
| Immunogen | KLH conjugated synthetic peptide derived from human SLC38A2/SNAT2 21-150/506 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | Amino acid transporter 2 Amino acid transporter A2 ATA2 SNAT2 KIAA1382 PRO1068 Protein 40-9-1 S38A2_HUMAN SAT2 Slc38a2 Sodium-coupled neutral amino acid transporter 2 Solute carrier family 38 member 2 System A amino acid transporter System A amino acid transporter 2 System A transporter 1 System N amino acid transporter 2. |
|---|---|
| Molecular Weight(MW) | 56 KDa |
| Cellular Localization | Cell membrane. Insulin promotes recruitment to the plasma membrane from a pool localized in the trans-Golgi network or endosomes. Enriched in the somatodendritic compartment of neurons, it is also detected at the axonal shaft but excluded from the nerve terminal. |
| SwissProt ID | Q96QD8 |
|---|
Application
Fig1: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-SLC38A2 (ER1916-78) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 56 kD
Application
Fig2: Sample: Intestine (Mouse) Lysate at 40 ug Primary: Anti-SLC38A2 (ER1916-78) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 56 kD
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SLC38A2) Polyclonal Antibody, Unconjugated (ER1916-78) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.| Positive Control | Ubiquitously expressed. Widely expressed in the central nervous system with higher concentrations in caudal regions. Expressed by glutamatergic and GABAergic neurons together with astrocytes and other non-neuronal cells in the cerebral cortex (at protein level). |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
