Application
Fig1: Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti- SLC4A4 (ER1916-82) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 116 kD Observed band size: 116 kD| Product Name | SLC4A4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DKFZp781H1314 Electrogenic sodium bicarbonate cotransporter 1 hhNMC HNBC 1 HNBC1 kNBC 1 KNBC kNBC1 Na(+)/HCO3(-) cotransporter Na+HCO3- cotransporter 4 NBC 1 NBC 2 NBC1 NBC2 Nbc4 NBCE 1 NBCE1 PNBC SLC4A5 Sodium bicarbonate cotransporter kidney sodium bicarbonate cotransporter member 4 Sodium bicarbonate cotransporter pancreas Solute carrier family 4 member 4 solute carrier family 4 sodium bicarbonate cotransporter member 4 Solute carrier family 4 sodium bicarbonate cotransporter member 5. |
| Product description | Electrogenic sodium/bicarbonate cotransporter with a Na(+):HCO3(-) stoichiometry varying from 1:2 to 1:3. May regulate bicarbonate influx/efflux at the basolateral membrane of cells and regulate intracellular pH. |
| Immunogen | KLH conjugated synthetic peptide derived from human electrogenic sodium bicarbonate cotransporter 1 isoform 2 1-100/1035 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatRabbit |
| Concentration | 1mg/ml |
| Alternative Names | DKFZp781H1314 Electrogenic sodium bicarbonate cotransporter 1 hhNMC HNBC 1 HNBC1 kNBC 1 KNBC kNBC1 Na(+)/HCO3(-) cotransporter Na+HCO3- cotransporter 4 NBC 1 NBC 2 NBC1 NBC2 Nbc4 NBCE 1 NBCE1 PNBC SLC4A5 Sodium bicarbonate cotransporter kidney sodium bicarbonate cotransporter member 4 Sodium bicarbonate cotransporter pancreas Solute carrier family 4 member 4 solute carrier family 4 sodium bicarbonate cotransporter member 4 Solute carrier family 4 sodium bicarbonate cotransporter member 5. |
|---|---|
| Molecular Weight(MW) | 116 KDa |
| Cellular Localization | Basolateral cell membrane; Multi-pass membrane protein. |
| SwissProt ID | O88343 |
|---|
Application
Fig1: Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti- SLC4A4 (ER1916-82) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 116 kD Observed band size: 116 kD| Positive Control | Isoform 1 is expressed in pancreas and to a lower extent in heart, skeletal muscle, liver, parotid salivary glands, prostate, colon, stomach, thyroid, brain and spinal chord. Corneal endothelium cells express only isoform 1 (at protein level). Isoform 2 is specifically expressed in kidney at the level of proximal tubules. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
