Application
Fig1: Sample: Thyroid gland (Rat) Lysate at 40 ug Primary: Anti-SLC5A8 (ER1916-83) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 67 kD Observed band size: 70 kDWB
(A) The NAD+ precursor transporter SLC12A8, SLC5A8, and SLC7A5 representative images of Western blot.| Product Name | SLC5A8 |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Acts as an electrogenic sodium (Na(+)) and chloride (Cl-)-dependent sodium-coupled solute transporter, including transport of monocarboxylates (short-chain fatty acids including L-lactate, D-lactate, pyruvate, acetate, propionate, valerate and butyrate), lactate, mocarboxylate drugs (nicotinate, benzoate, salicylate and 5-aminosalicylate) and ketone bodies (beta-D-hydroxybutyrate, acetoacetate and alpha-ketoisocaproate), with a Na(+):substrate stoichiometry of between 4:1 and 2:1. Catalyzes passive carrier mediated diffusion of iodide. Mediates iodide transport from the thyrocyte into the colloid lumen through the apical membrane. May be responsible for the absorption of D-lactate and monocarboxylate drugs from the intestinal tract. Acts as a tumor suppressor, suppressing colony formation in colon cancer, prostate cancer and glioma cell lines. May play a critical role in the entry of L-lactate and ketone bodies into neurons by a process driven by an electrochemical Na(+) gradient and hence contribute to the maintenance of the energy status and function of neurons. |
| Immunogen | KLH conjugated synthetic peptide derived from human SLC5A8 301-400/610 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | ChickenCowDogHumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | AIT Apical iodide transporter Electrogenic sodium monocarboxylate cotransporter SC5A8_HUMAN SLC5A8 SMCT SMCT1 sodium coupled monocarboxylate transporter 1 Sodium iodide related cotransporter Sodium iodide-related cotransporter Sodium-coupled monocarboxylate transporter 1 solute carrier family 5 iodide transporter member 8 Solute carrier family 5 member 8. |
|---|---|
| Molecular Weight(MW) | 67 KDa |
| Cellular Localization | Apical cell membrane; Multi-pass membrane protein. |
| SwissProt ID | Q8N695 |
|---|
Application
Fig1: Sample: Thyroid gland (Rat) Lysate at 40 ug Primary: Anti-SLC5A8 (ER1916-83) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 67 kD Observed band size: 70 kD
WB
(A) The NAD+ precursor transporter SLC12A8, SLC5A8, and SLC7A5 representative images of Western blot.| Positive Control | Expressed in normal thyroid, localized at the apical pole of thyroid cells facing the colloid lumen, but expression profoundly decreased in thyroid carcinomas. Expressed in normal colon but absent in colon aberrant crypt foci and colon cancers. Present in normal kidney cortex, brain, prostate, gastric mucosa and breast tissue but was significantly down-regulated in primary gliomas, gastric cancer, prostate tumors and breast tumors. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
