Application
Fig1: Sample: Brain (Rat) Lysate at 40 ug Primary: Anti-SLC7A5 (ER1916-84) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 55 kD Observed band size: 55 kDWB
(A) The NAD+ precursor transporter SLC12A8, SLC5A8, and SLC7A5 representative images of Western blot.| Product Name | SLC7A5 |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Involved in cellular amino acid uptake. Acts as an amino acid exchanger. Involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Plays a role in neuronal cell proliferation (neurogenesis) in brain. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. May play an important role in high-grade gliomas. Mediates blood-to-retina L-leucine transport across the inner blood-retinal barrier which in turn may play a key role in maintaining large neutral amino acids as well as neurotransmitters in the neural retina. Acts as the major transporter of tyrosine in fibroblasts. |
| Immunogen | KLH conjugated synthetic peptide derived from human SLC7A5 101-200/507 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | ChickenCowDogHumanMouseRabbitRat |
| Concentration | 1mg/ml |
| Alternative Names | LAT1 4F2 LC 4F2 light chain SLC7A5 4F2LC CD98 CD98 light chain CD98LC D16S469E DC49 E16 hLAT1 Integral membrane protein E16 L type amino acid transporter L type amino acid transporter 1 L-type amino acid transporter 1 Large neutral amino acids transporter 1 Large neutral amino acids transporter Large neutral amino acids transporter small subunit 1 LAT1_HUMAN Membrane protein E16 MPE16 Slc7a5 Sodium independent neutral amino acid transporter Sodium independent neutral amino acid transporter LAT1 Solute carrier family 7 (cationic amino acid transporter y+ system) member 5 Solute carrier family 7 member 5 TA1 Tumor associated protein 1 y+ system cationic amino acid transporter. |
|---|---|
| Molecular Weight(MW) | 55 KDa |
| Cellular Localization | Cytoplasm, cytosol. Apical cell membrane. Located to the plasma membrane by SLC3A2/4F2hc. Localized to the apical membrane of placental syncytiophoblastic cells. Expressed in both luminal and abluminal membranes of brain capillary endothelial cells. |
| SwissProt ID | Q01650 |
|---|
Application
Fig1: Sample: Brain (Rat) Lysate at 40 ug Primary: Anti-SLC7A5 (ER1916-84) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 55 kD Observed band size: 55 kD
WB
(A) The NAD+ precursor transporter SLC12A8, SLC5A8, and SLC7A5 representative images of Western blot.| Positive Control | Expressed abundantly in adult lung, liver, brain, skeletal muscle, placenta, bone marrow, testis, resting lymphocytes and monocytes, and in fetal liver. Weaker expression in thymus, cornea, retina, peripheral leukocytes, spleen, kidney, colon and lymph node. During gestation, expression in the placenta was significantly stronger at full-term than at the mid-trimester stage. Also expressed in all human tumor cell lines tested and in the astrocytic process of primary astrocytic gliomas. Expressed in retinal endothelial cells and in the intestinal epithelial cell line Caco-2. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
