Application
Fig1: Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-OATP-1/Slco1a1 Polyclonal Antibody, Unconjugated(ER1916-86) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig2: Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti-Slco1a1 (ER1916-86) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 74 kD Observed band size: 73 kDApplication
Fig3: Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti-Slco1a1 (ER1916-86) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 74 kD Observed band size: 74 kD| Product Name | Slco1a1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | OATP-1 Organic anion-transporting polypeptide 1 SO1A1_RAT Sodium-independent organic anion transporter 1 Solute carrier family 21 member 1 Solute carrier organic anion transporter family member 1A1. |
| Product description | Mediates the Na(+)-independent transport of organic anions such as bromosulfobromophthalein (BSP) and conjugated (taurocholate) and unconjugated (cholate) bile acids. |
| Immunogen | KLH conjugated synthetic peptide derived from rat Slco1a1 2-90/670 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Alternative Names | OATP-1 Organic anion-transporting polypeptide 1 SO1A1_RAT Sodium-independent organic anion transporter 1 Solute carrier family 21 member 1 Solute carrier organic anion transporter family member 1A1. |
|---|---|
| Molecular Weight(MW) | 74 KDa |
| Cellular Localization | Basolateral cell membrane |
| SwissProt ID | P46720 |
|---|
Application
Fig1: Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-OATP-1/Slco1a1 Polyclonal Antibody, Unconjugated(ER1916-86) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig2: Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti-Slco1a1 (ER1916-86) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 74 kD Observed band size: 73 kD
Application
Fig3: Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti-Slco1a1 (ER1916-86) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 74 kD Observed band size: 74 kD| Positive Control | Highly expressed in liver and kidney, and at lower levels in brain, lung, skeletal muscle and proximal colon. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
