Application
Fig1: Sample: Panc-1(Human) Cell Lysate at 30 ug Primary: Anti-SNF2H (ER1916-92) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 122 kD Observed band size: 127 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SNF2H) Polyclonal Antibody, Unconjugated (ER1916-92) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SNF2H) Polyclonal Antibody, Unconjugated (ER1916-92) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | SNF2H |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 4933427E24Rik ATP-dependent chromatin remodeling protein SNF2H D030040M08Rik D330027N15Rik EC 3.6.1.- hISWI hSNF2H ISWI MommeD4 SMARCA5 SMCA5 SMCA5_HUMAN Snf2h Sucrose nonfermenting like 5 Sucrose nonfermenting protein 2 homolog Sucrose nonfermenting, yeast, homolog of sucrose nonfermenting-like 5 SWI/SNF related matrix associated actin dependent regulator of chromatin a5 SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily a member 5 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5 WCRF135. |
| Product description | Helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity. Complexes containing SMARCA5 are capable of forming ordered nucleosome arrays on chromatin; this may require intact histone H4 tails. Also required for replication of pericentric heterochromatin in S-phase specifically in conjunction with BAZ1A. Probably plays a role in repression of polI dependent transcription of the rDNA locus, through the recruitment of the SIN3/HDAC1 corepressor complex to the rDNA promoter. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. Essential component of the NoRC (nucleolar remodeling complex) complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing. |
| Immunogen | KLH conjugated synthetic peptide derived from human SNF2H 901-1000/1052 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbitZebra FishSheepGuinea Pig |
| Concentration | 1mg/ml |
| Alternative Names | 4933427E24Rik
ATP-dependent chromatin remodeling protein SNF2H
D030040M08Rik
D330027N15Rik
EC 3.6.1.-
hISWI
hSNF2H
ISWI
MommeD4
SMARCA5
SMCA5
SMCA5_HUMAN
Snf2h
Sucrose nonfermenting like 5
Sucrose nonfermenting protein 2 homolog
Sucrose nonfermenting yeast homolog of sucrose nonfermenting-like 5 SWI/SNF related matrix associated actin dependent regulator of chromatin a5 SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily a member 5 SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily a member 5 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5 WCRF135. |
|---|---|
| Molecular Weight(MW) | 122 KDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | O60264 |
|---|
Application
Fig1: Sample: Panc-1(Human) Cell Lysate at 30 ug Primary: Anti-SNF2H (ER1916-92) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 122 kD Observed band size: 127 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SNF2H) Polyclonal Antibody, Unconjugated (ER1916-92) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SNF2H) Polyclonal Antibody, Unconjugated (ER1916-92) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | Ubiquitously expressed. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
