Application
Fig1: Sample: Brain(Rat) lysate at 30ug; Liver(Rat) lysate at 30ug; Primary: Anti-NHE1 (ER1916-95) at 1:200 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000 dilution; Predicted band size : 91kD Observed band size : 91kD We are unsure as to the identity of these extra bandsApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Sodium hydrogen exchanger 1) Polyclonal Antibody, Unconjugated (ER1916-95) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig3: Sample: molt-4(human)cell Lysate at 40 ug Primary: Anti- Sodium hydrogen exchanger 1 (ER1916-95) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 91kD Observed band size: 91 kD| Product Name | Sodium hydrogen exchanger 1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | amiloride-sensitive APNH APNH1 FLJ42224 Na Li countertransporter Na(+)/H(+) antiporter Na(+)/H(+) exchanger 1 Na+ H+ antiporter amiloride-sensitive Na+ H+ antiporter Na+ H+ exchanger 1 NHE-1 NHE1 OTTHUMP00000004468 SL9A1_HUMAN SLC9A1 Sodium hydrogen exchanger 1 Sodium/hydrogen exchanger 1 solute carrier family 9 Solute carrier family 9 member 1 Solute carrier family 9 sodium hydrogen exchanger isoform 1 antiporter Na+ H+ amiloride sensitive Solute carrier family 9 subfamily A (NHE1 cation proton antiporter 1) member 1 Solute carrier family 9 subfamily A member 1. |
| Product description | Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction. |
| Immunogen | KLH conjugated synthetic peptide derived from human NHE1 470-550/815 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | amiloride-sensitive APNH APNH1 FLJ42224 Na Li countertransporter Na(+)/H(+) antiporter Na(+)/H(+) exchanger 1 Na+ H+ antiporter amiloride-sensitive Na+ H+ antiporter Na+ H+ exchanger 1 NHE-1 NHE1 OTTHUMP00000004468 SL9A1_HUMAN SLC9A1 Sodium hydrogen exchanger 1 Sodium/hydrogen exchanger 1 solute carrier family 9 Solute carrier family 9 member 1 Solute carrier family 9 sodium hydrogen exchanger isoform 1 antiporter Na+ H+ amiloride sensitive Solute carrier family 9 subfamily A (NHE1 cation proton antiporter 1) member 1 Solute carrier family 9 subfamily A member 1. |
|---|---|
| Molecular Weight(MW) | 91 KDa |
| Cellular Localization | Membrane; Multi-pass membrane protein. |
| SwissProt ID | P19634 |
|---|
Application
Fig1: Sample: Brain(Rat) lysate at 30ug; Liver(Rat) lysate at 30ug; Primary: Anti-NHE1 (ER1916-95) at 1:200 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000 dilution; Predicted band size : 91kD Observed band size : 91kD We are unsure as to the identity of these extra bands
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Sodium hydrogen exchanger 1) Polyclonal Antibody, Unconjugated (ER1916-95) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig3: Sample: molt-4(human)cell Lysate at 40 ug Primary: Anti- Sodium hydrogen exchanger 1 (ER1916-95) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 91kD Observed band size: 91 kD| Positive Control | Kidney and intestine. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
