Application
Fig1: Sample: Testis (Mouse) Lysate at 40 ug Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-SOX4 (ER1916-97) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SIAE) Polyclonal Antibody, Unconjugated (bs-17480R) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SOX4) Polyclonal Antibody, Unconjugated (ER1916-97) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | SOX4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SOX4 SOX 4 SOX-4 Ecotropic viral integration site 16 EVI 16 EVI16 SRY (sex determining region Y) box 4 SRY box containing gene 4 SRY related HMG box gene 4 SOX4_HUMAN. |
| Product description | The SOX (SRY-box containing gene) gene family encodes a group of transcription factors defined by the conserved high motility group (HMG) DNA-binding domain. They are involved in the regulation of embryonic development and in the determination of cell fate. SOX4 shows a high degree of sequence homology with SOX11. Both are expressed in the CNS. SOX4 and SOX11 expression increase together in the developing CNS (with some exceptions: external granule layer of the cerebellum). SOX4 knock-out mice have been shown to die of a heart defect half way through gestation with no observable CNS phenotype, although it has been proposed that SOX11 compensates for the absence of SOX4. |
| Immunogen | KLH conjugated synthetic peptide derived from human SOX4 54-103/474 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChicken |
| Concentration | 1mg/ml |
| Alternative Names | SOX4 SOX 4 SOX-4 Ecotropic viral integration site 16 EVI 16 EVI16 SRY (sex determining region Y) box 4 SRY box containing gene 4 SRY related HMG box gene 4 SOX4_HUMAN. |
|---|---|
| Molecular Weight(MW) | 52 KDa |
| Cellular Localization | Nuclear |
| SwissProt ID | Q06945 |
|---|
Application
Fig1: Sample: Testis (Mouse) Lysate at 40 ug Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-SOX4 (ER1916-97) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SIAE) Polyclonal Antibody, Unconjugated (bs-17480R) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SOX4) Polyclonal Antibody, Unconjugated (ER1916-97) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | Testis, brain, and heart. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
