Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-SPARCL1 (ER1917-01) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 74 kD Observed band size: 73 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (SPARCL1) Polyclonal Antibody, Unconjugated (ER1917-01) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.| Product Name | SPARCL1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ecm2 SPRL1 RAGS1 antigen Extracellular matrix protein 2 Hevin High endothelial venule protein MAST 9 mast9 Matrix glycoprotein Sc1 PIG33 proliferation inducing protein 33 SC1 SPARC like 1 SPARC-like protein 1 SPRL1_HUMAN. |
| Immunogen | KLH conjugated synthetic peptide derived from human SPARCL1/Ecm2 601-664/664 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatPigHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Ecm2 SPRL1 RAGS1 antigen Extracellular matrix protein 2 Hevin High endothelial venule protein MAST 9 mast9 Matrix glycoprotein Sc1 PIG33 proliferation inducing protein 33 SC1 SPARC like 1 SPARC-like protein 1 SPRL1_HUMAN. |
|---|---|
| Molecular Weight(MW) | 74 KDa |
| Cellular Localization | Secreted, extracellular space, extracellular matrix. |
| SwissProt ID | Q14515 |
|---|
Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-SPARCL1 (ER1917-01) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 74 kD Observed band size: 73 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (SPARCL1) Polyclonal Antibody, Unconjugated (ER1917-01) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.| Positive Control | Highly expressed in lymph node, brain, heart, lung, skeletal muscle, ovary, small intestine, and colon, with lower levels in placenta, pancreas, testis, spleen, and thymus, and no expression in kidney, liver, and peripheral blood leukocytes. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
