Application
Fig1: Sample: HL60(Human) Cell Lysate at 30 ug NIH/3T3(Mouse) Cell Lysate at 30 ug U2OS(Human) Cell Lysate at 30 ug HL60 (Human) Cell Lysate at 30 ug Primary: Anti- SPECC1L (ER1917-08) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 125 kD Observed band size: 125 kD| Product Name | SPECC1L |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cytokinesis and spindle organization A Cytospin-A CYTSA CYTSA_HUMAN Renal carcinoma antigen NY-REN-22 SPECC1-like protein specc1l Sperm antigen with calponin homology and coiled-coil domains 1-like. |
| Product description | Involved in cytokinesis and spindle organization. May play a role in actin cytoskeleton organization and microtubule stabilization and hence required for proper cell adhesion and migration. |
| Immunogen | KLH conjugated synthetic peptide derived from human SPECC1L 161-260/1117 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | Cytokinesis and spindle organization A Cytospin-A CYTSA CYTSA_HUMAN Renal carcinoma antigen NY-REN-22 SPECC1-like protein specc1l Sperm antigen with calponin homology and coiled-coil domains 1-like. |
|---|---|
| Molecular Weight(MW) | 125 KDa |
| Cellular Localization | Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle. Cell junction, gap junction. Note=Colocalizes with acetylated alpha-tubulin, gamma-tubulin and F-actin. Also observed in a ring around gamma-tubulin containing centrioles possibly in the microtubule organizing center. |
| SwissProt ID | Q69YQ0 |
|---|
Application
Fig1: Sample: HL60(Human) Cell Lysate at 30 ug NIH/3T3(Mouse) Cell Lysate at 30 ug U2OS(Human) Cell Lysate at 30 ug HL60 (Human) Cell Lysate at 30 ug Primary: Anti- SPECC1L (ER1917-08) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 125 kD Observed band size: 125 kD| Application Notes | WB:1:500-2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
