Application
Fig1: Sample: Lymph node (Mouse) Lysate at 40 ug Primary: Anti-SPIB (ER1917-11) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 29 kDApplication
Fig2: Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti-SPIB (ER1917-11) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 29 kD| Product Name | SPIB |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPI B Spi B transcription factor Spib SPIB_HUMAN Transcription factor Spi-B. |
| Product description | Sequence specific transcriptional activator which binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Promotes development of plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells. These cells have the capacity to produce large amounts of interferon and block viral replication. May be required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation. |
| Immunogen | KLH conjugated synthetic peptide derived from human SPIB 181-262/262 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | SPI B Spi B transcription factor Spib SPIB_HUMAN Transcription factor Spi-B. |
|---|---|
| Molecular Weight(MW) | 29 KDa |
| Cellular Localization | Cytoplasm and Nucleus. |
| SwissProt ID | Q01892 |
|---|
Application
Fig1: Sample: Lymph node (Mouse) Lysate at 40 ug Primary: Anti-SPIB (ER1917-11) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 29 kD
Application
Fig2: Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti-SPIB (ER1917-11) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 29 kD| Positive Control | Expressed in plasmacytoid dendritic cells (pDCs) and B-cells, not expressed in T-cells or granulocytes. May also be enriched in stem cell populations of the liver. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
