Application
Fig1: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Squalene Epoxidase Polyclonal Antibody, Unconjugated(ER1917-17) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig2: Sample: NIH/3T3(Mouse) Cell Lysate at 30 ug A549(Human) Cell Lysate at 30 ug Primary: Anti-Squalene Epoxidase (ER1917-17) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kD Observed band size: 64 kD| Product Name | Squalene Epoxidase |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ERG1 SE antibody SQLE Squalene monooxygenase ERG1_HUMAN. |
| Product description | Catalyzes the first oxygenation step in sterol biosynthesis and is suggested to be one of the rate-limiting enzymes in this pathway. |
| Immunogen | KLH conjugated synthetic peptide derived from human Squalene Epoxidase 101-200/574 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | ERG1 SE antibody SQLE Squalene monooxygenase ERG1_HUMAN. |
|---|---|
| Molecular Weight(MW) | 64 KDa |
| Cellular Localization | Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. |
| SwissProt ID | Q14534 |
|---|
Application
Fig1: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Squalene Epoxidase Polyclonal Antibody, Unconjugated(ER1917-17) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig2: Sample: NIH/3T3(Mouse) Cell Lysate at 30 ug A549(Human) Cell Lysate at 30 ug Primary: Anti-Squalene Epoxidase (ER1917-17) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kD Observed band size: 64 kD| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
