Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SREBP1) Polyclonal Antibody, Unconjugated (ER1917-19) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: Lung(Mouse) Lysate at 30 ug Ovary (Mouse) Lysate at 30 ug Skin (Mouse) Lysate at 30 ug Primary: Anti- SREBP1 (ER1917-19) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54/126 kD Observed band size: 50 kDApplication
Fig3: Sample: Muscle(Mouse) Lysate at 30 ug Primary: Anti- SREBP1 (ER1917-19) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54/126 kD Observed band size: 50 kDApplication
Fig4: Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SREBP-1) Polyclonal Antibody, Unconjugated (ER1917-19) at 1:200 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Application
citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SREBP-1) Polyclonal Antibody, Unconjugated (ER1917-19) at 1:200 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Application
Fig6: Sample: Liver(Mouse) Cell Lysate at 40 ug Liver(Rat) Cell Lysate at 40 ug Primary: Anti-SREBP1 (ER1917-19) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54/126 kD Observed band size: 54 kD| Product Name | SREBP1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Sterol regulatory element binding protein 1 ADD 1 BHLHD1 D630008H06 SREBF 1 SREBP-1 SREBF1 SREBP 1 SREBP1 SREBP 1c Sterol regulatory element binding protein 1 Sterol Regulatory Element Binding Transcription Factor 1 / Protein 1 Sterol regulatory element binding transcription factor 1 SRBP1_HUMAN Sterol regulatory element-binding protein 1 SREBP-1 Class D basic helix-loop-helix protein 1 bHLHd1 Sterol regulatory element-binding transcription factor 1 Processed sterol regulatory element-binding protein 1. |
| Product description | This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. This gene is located within the Smith-Magenis syndrome region on chromosome 17. |
| Immunogen | KLH conjugated synthetic peptide derived from human SREBP-1 301-450/1147 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChicken |
| Concentration | 1mg/ml |
| Alternative Names | Sterol regulatory element binding protein 1 ADD 1 BHLHD1 D630008H06 SREBF 1 SREBP-1 SREBF1 SREBP 1 SREBP1 SREBP 1c Sterol regulatory element binding protein 1 Sterol Regulatory Element Binding Transcription Factor 1 / Protein 1 Sterol regulatory element binding transcription factor 1 SRBP1_HUMAN Sterol regulatory element-binding protein 1 SREBP-1 Class D basic helix-loop-helix protein 1 bHLHd1 Sterol regulatory element-binding transcription factor 1 Processed sterol regulatory element-binding protein 1. |
|---|---|
| Molecular Weight(MW) | 54/126 KDa |
| Cellular Localization | Endoplasmic reticulum membrane. Nucleus. |
| SwissProt ID | P36956 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SREBP1) Polyclonal Antibody, Unconjugated (ER1917-19) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: Lung(Mouse) Lysate at 30 ug Ovary (Mouse) Lysate at 30 ug Skin (Mouse) Lysate at 30 ug Primary: Anti- SREBP1 (ER1917-19) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54/126 kD Observed band size: 50 kD
Application
Fig3: Sample: Muscle(Mouse) Lysate at 30 ug Primary: Anti- SREBP1 (ER1917-19) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54/126 kD Observed band size: 50 kD
Application
Fig4: Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SREBP-1) Polyclonal Antibody, Unconjugated (ER1917-19) at 1:200 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Application
citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SREBP-1) Polyclonal Antibody, Unconjugated (ER1917-19) at 1:200 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Application
Fig6: Sample: Liver(Mouse) Cell Lysate at 40 ug Liver(Rat) Cell Lysate at 40 ug Primary: Anti-SREBP1 (ER1917-19) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54/126 kD Observed band size: 54 kD| Positive Control | Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
