Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SRF) Polyclonal Antibody, Unconjugated (ER1917-20) at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Application
Fig2: Sample: A431(Human) Cell Lysate at 30 ug Hela(Human) Cell Lysate at 30 ug 293T(Human) Cell Lysate at 30 ug Primary: Anti- SRF (ER1917-20) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 52 kDApplication
Fig3: Sample: NIH/3T3 Cell Lysate at 30 ug Primary: Anti-SRF (ER1917-20) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution Predicted band size: 56 kD Observed band size: 54 kDApplication
Fig4: Sample: Muscle(Mouse) Lysate at 30 ug Primary: Anti- SRF (ER1917-20) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution Predicted band size: 56 kD Observed band size: 56 kDApplication
Fig5: Sample: Large intestine (Mouse) Lysate at 30 ug Primary: Anti- SRF (ER1917-20) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution Predicted band size: 56 kD Observed band size: 54 kD| Product Name | SRF |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | c fos serum response element binding factor Serum Response Factor SRF c fos serum response element binding transcription factor ELK3 ERP MCM 1 MCM-1 MCM1 OTTHUMP00000039820 SAP2 Serum response factor SRF SRF serum response factor c fos serum response element binding transcription factor SRF_HUMAN. |
| Product description | SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation. |
| Immunogen | KLH conjugated synthetic peptide derived from human SRF 101-250/508 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCow |
| Concentration | 1mg/ml |
| Alternative Names | c fos serum response element binding factor Serum Response Factor SRF c fos serum response element binding transcription factor ELK3 ERP MCM 1 MCM-1 MCM1 OTTHUMP00000039820 SAP2 Serum response factor SRF SRF serum response factor c fos serum response element binding transcription factor SRF_HUMAN. |
|---|---|
| Molecular Weight(MW) | 56 KDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | P11831 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SRF) Polyclonal Antibody, Unconjugated (ER1917-20) at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Application
Fig2: Sample: A431(Human) Cell Lysate at 30 ug Hela(Human) Cell Lysate at 30 ug 293T(Human) Cell Lysate at 30 ug Primary: Anti- SRF (ER1917-20) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 52 kD
Application
Fig3: Sample: NIH/3T3 Cell Lysate at 30 ug Primary: Anti-SRF (ER1917-20) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution Predicted band size: 56 kD Observed band size: 54 kD
Application
Fig4: Sample: Muscle(Mouse) Lysate at 30 ug Primary: Anti- SRF (ER1917-20) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution Predicted band size: 56 kD Observed band size: 56 kD
Application
Fig5: Sample: Large intestine (Mouse) Lysate at 30 ug Primary: Anti- SRF (ER1917-20) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution Predicted band size: 56 kD Observed band size: 54 kD| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
