Application
Fig1: Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti-SRXN1 (ER1917-21) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 15 kD Observed band size: 15 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by microwave in sodium citratebuffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3%BSA) at RTfor 30min; Antibody incubation with (SRXN1) Polyclonal/MonoclonalAntibody, Unconjugated (ER1917-21) at 1:400 overnight at 4℃,followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.| Product Name | SRXN1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C20orf1392 Chromosome 20 open reading frame 139 dJ850E9.2 Npn3 Npn31 SRX1 SRXN 1 Sulfiredoxin 1 Sulfiredoxin 1 homolog (S. cerevisiae) Sulfiredoxin 1 homolog YKL086W SRXN1_HUMAN. |
| Product description | SRXN1 contributes to oxidative stress resistance by reducing cysteine-sulfinic acid formed under exposure to oxidants in the peroxiredoxins PRDX1, PRDX2, PRDX3 and PRDX4. It does not act on PRDX5 or PRDX6. SRXN1 may catalyze the reduction in a multi-step process by acting both as a specific phosphotransferase and a thioltransferase. |
| Immunogen | KLH conjugated synthetic peptide derived from human SRXN1 45-137/137 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | C20orf1392 Chromosome 20 open reading frame 139 dJ850E9.2 Npn3 Npn31 SRX1 SRXN 1 Sulfiredoxin 1 Sulfiredoxin 1 homolog (S. cerevisiae) Sulfiredoxin 1 homolog YKL086W SRXN1_HUMAN. |
|---|---|
| Molecular Weight(MW) | 15 KDa |
| Cellular Localization | Cytoplasmic |
| SwissProt ID | Q9BYN0 |
|---|
Application
Fig1: Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti-SRXN1 (ER1917-21) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 15 kD Observed band size: 15 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by microwave in sodium citratebuffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3%BSA) at RTfor 30min; Antibody incubation with (SRXN1) Polyclonal/MonoclonalAntibody, Unconjugated (ER1917-21) at 1:400 overnight at 4℃,followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.| Positive Control | Widely expressed with highest levels in kidney, lung, spleen and thymus. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
