Application
Fig1: Sample: U87MG(Human) Cell Lysate at 30 ug Primary: Anti- ST18 (ER1917-22) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 115 kD Observed band size: 115 kDApplication
Fig2: Blank control: Mouse kidney. Primary Antibody (green line): Rabbit Anti-ST18 antibody (ER1917-22) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Product Name | ST18 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KIAA0535 St18 ST18_HUMAN suppression of tumorigenicity 18 (breast carcinoma) (zinc finger protein) Suppression of tumorigenicity 18 protein Suppression of tumorigenicity protein 18 Zinc finger protein 387 Znf387. |
| Product description | Repressor that binds to DNA sequences containing a bipartite element consisting of a direct repeat of the sequence 5'-AAAGTTT-3' separated by 2-9 nucleotides. Represses basal transcription activity from target promoters (By similarity). Inhibits colony formation in cultured breast cancer cells. |
| Immunogen | KLH conjugated synthetic peptide derived from human ST18/Znf387 451-550/1047 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:3ug/Test: |
|
| Species Reactivity | HumanMouseRatDogHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | KIAA0535 St18 ST18_HUMAN suppression of tumorigenicity 18 (breast carcinoma) (zinc finger protein) Suppression of tumorigenicity 18 protein Suppression of tumorigenicity protein 18 Zinc finger protein 387 Znf387. |
|---|---|
| Molecular Weight(MW) | 115 KDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | O60284 |
|---|
Application
Fig1: Sample: U87MG(Human) Cell Lysate at 30 ug Primary: Anti- ST18 (ER1917-22) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 115 kD Observed band size: 115 kD
Application
Fig2: Blank control: Mouse kidney. Primary Antibody (green line): Rabbit Anti-ST18 antibody (ER1917-22) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Positive Control | Detected at low levels in heart, liver, kidney, skeletal muscle, pancreas, testis, ovary and prostate. Detected at even lower levels in mammary epithelial cells and breast cancer cells. |
|---|---|
| Application Notes | WB:1:500-2000: FC:3ug/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
