Application
Fig1: Blank control (blue line): Hela (fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-StAR antibody (ER1917-23),Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.Application
Fig2: Sample: Ovary (Mouse) Lysate at 30 ug Brain (Mouse) Lysate at 30 ug Primary: Anti-STAR/STARD1(ER1917-23) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution; Predicted band size : 32kD Observed band size : 32kDApplication
Fig3: Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (StAR) Polyclonal Antibody, Unconjugated (ER1917-23) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.Application
Fig4: Sample: Adrenal gland(mouse) Lysate at 40 ug Primary: Anti-StAR (ER1917-23) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32kD Observed band size: 32 kDApplication
Fig5: Sample: K562(human) cell Lysate at 30 ug Primary: Anti-StAR (ER1917-23) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32kD Observed band size: 32 kD| Product Name | StAR |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | StARD1 Cholesterol trafficker Luteinizing hormone induced protein Mitochondrial steroid acute regulatory protein StAR related lipid transfer (START) domain containing 1 StARD1 START domain containing protein 1 Steroidogenic Acute Regulatory Protein Steroidogenic acute regulatory protein mitochondrial STAR_HUMAN. |
| Product description | The protein encoded by this gene plays a key role in the acute regulation of steroid hormone synthesis by enhancing the conversion of cholesterol into pregnenolone. This protein permits the cleavage of cholesterol into pregnenolone by mediating the transport of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane. Mutations in this gene are a cause of congenital lipoid adrenal hyperplasia (CLAH), also called lipoid CAH. A pseudogene of this gene is located on chromosome 13. |
| Immunogen | KLH conjugated synthetic peptide derived from human StAR/StARD1 101-200/285 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:3μg /Test: |
|
| Species Reactivity | HumanMouseRatDogCowHorse |
| Concentration | 1mg/ml |
| Alternative Names | StARD1 Cholesterol trafficker Luteinizing hormone induced protein Mitochondrial steroid acute regulatory protein StAR related lipid transfer (START) domain containing 1 StARD1 START domain containing protein 1 Steroidogenic Acute Regulatory Protein Steroidogenic acute regulatory protein mitochondrial STAR_HUMAN. |
|---|---|
| Molecular Weight(MW) | 32 KDa |
| Cellular Localization | Mitochondrion. |
| SwissProt ID | P49675 |
|---|
Application
Fig1: Blank control (blue line): Hela (fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-StAR antibody (ER1917-23),Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.
Application
Fig2: Sample: Ovary (Mouse) Lysate at 30 ug Brain (Mouse) Lysate at 30 ug Primary: Anti-STAR/STARD1(ER1917-23) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution; Predicted band size : 32kD Observed band size : 32kD
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (StAR) Polyclonal Antibody, Unconjugated (ER1917-23) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Application
Fig4: Sample: Adrenal gland(mouse) Lysate at 40 ug Primary: Anti-StAR (ER1917-23) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32kD Observed band size: 32 kD
Application
Fig5: Sample: K562(human) cell Lysate at 30 ug Primary: Anti-StAR (ER1917-23) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32kD Observed band size: 32 kD| Positive Control | Hela, Ovary (Mouse) Lysate, Brain (Mouse) Lysate, Rat testis, Adrenal gland(mouse) Lysate, K562(human) cell Lysate. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:3μg /Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
