Application
Fig1: Sample: A549(Human) Cell Lysate at 30 ug Primary: Anti-STEAP1 (ER1917-24) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kD Observed band size: 40 kDApplication
Fig2: Tissue/cell: human laryngocarcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-STEAP1 Polyclonal Antibody, Unconjugated(ER1917-24) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Product Name | STEAP1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Six transmbrane epithelial antigen of the prostate MGC19484 PRSS 24 PRSS24 Six transmembrane epithelial antigen of the prostate STEAP 1 STEAP1 STEA1_HUMAN. |
| Product description | This gene is predominantly expressed in prostate tissue, and is found to be upregulated in multiple cancer cell lines. The gene product is predicted to be a six-transmembrane protein, and was shown to be a cell surface antigen significantly expressed at cell-cell junctions. Metalloreductase that has the ability to reduce both Fe(3+) to Fe(2+) and Cu(2+) to Cu(1+). Uses NAD(+) as acceptor. |
| Immunogen | KLH conjugated synthetic peptide derived from human STEAP1 251-339/339 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatCowHorse |
| Concentration | 1mg/ml |
| Alternative Names | Six transmbrane epithelial antigen of the prostate MGC19484 PRSS 24 PRSS24 Six transmembrane epithelial antigen of the prostate STEAP 1 STEAP1 STEA1_HUMAN. |
|---|---|
| Molecular Weight(MW) | 40 KDa |
| Cellular Localization | Endosome membrane; Multi-pass membrane protein. |
| SwissProt ID | Q9UHE8 |
|---|
Application
Fig1: Sample: A549(Human) Cell Lysate at 30 ug Primary: Anti-STEAP1 (ER1917-24) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kD Observed band size: 40 kD
Application
Fig2: Tissue/cell: human laryngocarcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-STEAP1 Polyclonal Antibody, Unconjugated(ER1917-24) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Positive Control | Ubiquitously expressed. Highly expressed in prostate tumors. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
