Application
Fig1: Sample: HepG2(Human) Cell Lysate at 30 ug U2OS(Human) Cell Lysate at 30 ug Primary: Anti- STEP (ER1917-25) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 63 kD Observed band size: 63 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (STEP) Polyclonal Antibody, Unconjugated (ER1917-25) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.| Product Name | STEP |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PTPN5 Neural specific protein tyrosine phosphatase Neural-specific protein-tyrosine phosphatase Protein tyrosine phosphatase non receptor type 5 (striatum enriched) Protein tyrosine phosphatase non receptor type 5 Protein tyrosine phosphatase striatum enriched PTN5 PTN5_HUMAN PTP STEP PTPN 5 Ptpn5 PTPSTEP Striatum-enriched protein-tyrosine phosphatase Tyrosine protein phosphatase non receptor type 5 Tyrosine-protein phosphatase non-receptor type 5 FLJ14427. |
| Product description | May regulates the activity of several effector molecules involved in synaptic plasticity and neuronal cell survival, including MAPKs, Src family kinases and NMDA receptors. |
| Immunogen | KLH conjugated synthetic peptide derived from human PTPN5 201-300/565 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatRabbit |
| Concentration | 1mg/ml |
| Alternative Names | PTPN5 Neural specific protein tyrosine phosphatase Neural-specific protein-tyrosine phosphatase Protein tyrosine phosphatase non receptor type 5 (striatum enriched) Protein tyrosine phosphatase non receptor type 5 Protein tyrosine phosphatase striatum enriched PTN5 PTN5_HUMAN PTP STEP PTPN 5 Ptpn5 PTPSTEP Striatum-enriched protein-tyrosine phosphatase Tyrosine protein phosphatase non receptor type 5 Tyrosine-protein phosphatase non-receptor type 5 FLJ14427. |
|---|---|
| Molecular Weight(MW) | 63 KDa |
| Cellular Localization | Cellular localizationEndoplasmic reticulum membrane. |
| SwissProt ID | P54829 |
|---|
Application
Fig1: Sample: HepG2(Human) Cell Lysate at 30 ug U2OS(Human) Cell Lysate at 30 ug Primary: Anti- STEP (ER1917-25) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 63 kD Observed band size: 63 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (STEP) Polyclonal Antibody, Unconjugated (ER1917-25) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
