Application
Fig1: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-STK40 (ER1917-29) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 48 kD Observed band size: 48 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (STK40) Polyclonal Antibody, Unconjugated (ER1917-29) at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig3: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti- STK40 (ER1917-29) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 49 kD| Product Name | STK40 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ser/Thr like kinase Serine/threonine kinase 40 Serine/threonine protein kinase 40 SgK495 SHIK SINK homologous serine/threonine protein kinase SINK-homologous serine/threonine-protein kinase STK 40 Sugen kinase 495 STK40_HUMAN. |
| Product description | May be a negative regulator of NF-kappa-B and p53-mediated gene transcription. |
| Immunogen | KLH conjugated synthetic peptide derived from human STK40 231-330/435 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Ser/Thr like kinase Serine/threonine kinase 40 Serine/threonine protein kinase 40 SgK495 SHIK SINK homologous serine/threonine protein kinase SINK-homologous serine/threonine-protein kinase STK 40 Sugen kinase 495 STK40_HUMAN. |
|---|---|
| Molecular Weight(MW) | 48 KDa |
| Cellular Localization | Cytoplasmic and Nuclear. |
| SwissProt ID | Q8N2I9 |
|---|
Application
Fig1: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-STK40 (ER1917-29) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 48 kD Observed band size: 48 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (STK40) Polyclonal Antibody, Unconjugated (ER1917-29) at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig3: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti- STK40 (ER1917-29) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 49 kD| Positive Control | Strongly expressed in heart, brain, placenta, lung, skeletal muscle, kidney, spleen, thymus, prostate, liver, pancreas, testis, ovary, small intestine, colon and peripheral blood leukocytes. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
