Application
Fig1: Sample: Hela(Human) Cell Lysate at 30 ug Siha(Human) Cell Lysate at 30 ug Primary: Anti-STOML2 (ER1917-30) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 39 kD Observed band size: 39 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (STOML2) Polyclonal Antibody, Unconjugated (ER1917-30) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | STOML2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EPB72 like protein 2 HSPC108 OTTHUMP00000021320 SLP 2 SLP2 SLP-2 Stomatin (EPB72) like 2 Stomatin like 2 Stomatin like protein 2 STOML2 STML2_HUMAN. |
| Product description | STOML2 is similar in sequence to stomatin. It is a 356 amino acid protein with a calculated molecular mass of 38.5 kDa. STOML2 has 3 potential initiator sites, all sharing the same open reading frame. The STOML2 protein contains the cognate stomatin family consensus sequence, but it lacks the characteristic N-terminal hydrophobic domain and palmitoylation consensus sequence. STOML2 shares greatest sequence homology with stomatin and SLP1 in a region predicted to contain beta sheet and alpha helix structures. Northern blot analysis detected a 1.5 kb STOML2 transcript in all tissues examined, with highest levels in heart, liver, and pancreas. Western blot analysis detected STOML2 at apparent molecular masses of 45.5 kDa or 44.6 kDa in all human and mammalian cell lines and tissues examined, including red blood cells. Some cells also showed a faint band at about 34.3 kDa, which may represent translation from an alternate initiation site. |
| Immunogen | KLH conjugated synthetic peptide derived from human STOML2/SLP2 251-356/356 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | EPB72 like protein 2 HSPC108 OTTHUMP00000021320 SLP 2 SLP2 SLP-2 Stomatin (EPB72) like 2 Stomatin like 2 Stomatin like protein 2 STOML2 STML2_HUMAN. |
|---|---|
| Molecular Weight(MW) | 39 KDa |
| Cellular Localization | Membrane; peripheral membrane protein. Cytoplasm. Associated with the cytoskeleton. |
| SwissProt ID | Q9UJZ1 |
|---|
Application
Fig1: Sample: Hela(Human) Cell Lysate at 30 ug Siha(Human) Cell Lysate at 30 ug Primary: Anti-STOML2 (ER1917-30) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 39 kD Observed band size: 39 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (STOML2) Polyclonal Antibody, Unconjugated (ER1917-30) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | Ubiquitously expressed at low levels. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
