Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Stra8) Polyclonal Antibody, Unconjugated (ER1917-31) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: Testis (Rat) Lysate at 40 ug Primary: Anti-ER1917-31 (ER1917-31) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 45 kDApplication
Fig3: Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti-ER1917-31 (ER1917-31) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 45 kD| Product Name | Stra8 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Stimulated by retinoic acid Gene 8 STRA8_HUMAN Stimulated by retinoic acid gene 8 protein homolog STRA 8 STRA8. |
| Product description | Required for the transition into meiosis for both female and male germ cells. In female germ cells, required for premeiotic DNA replication and subsequent events in meiotic prophase |
| Immunogen | KLH conjugated synthetic peptide derived from human Stra8 201-300/330 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Stimulated by retinoic acid Gene 8 STRA8_HUMAN Stimulated by retinoic acid gene 8 protein homolog STRA 8 STRA8. |
|---|---|
| Molecular Weight(MW) | 45 KDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | Q7Z7C7 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Stra8) Polyclonal Antibody, Unconjugated (ER1917-31) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: Testis (Rat) Lysate at 40 ug Primary: Anti-ER1917-31 (ER1917-31) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 45 kD
Application
Fig3: Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti-ER1917-31 (ER1917-31) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 45 kD| Positive Control | Expressed specifically in testis. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
