Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Styk1) Polyclonal Antibody, Unconjugated (ER1917-32) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: Small intestine (Mouse) Lysate at 40 ug Primary: Anti-Styk1 (ER1917-32) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 48 kD| Product Name | Styk1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NOK Novel oncogene with kinase domain Protein kinase STYK1 Protein PK unique Protein PK-unique Serine/threonine/tyrosine kinase 1 Styk 1 STYK1 STYK1_HUMAN SuRTK 106 SuRTK106 Tyrosine protein kinase STYK1 Tyrosine-protein kinase STYK1. |
| Product description | Probable tyrosine protein-kinase, which has strong transforming capabilities on a variety of cell lines. When overexpressed, it can also induce tumor cell invasion as well as metastasis in distant organs. May act by activating both MAP kinase and phosphatidylinositol 3'-kinases (PI3K) pathways (By similarity). |
| Immunogen | KLH conjugated synthetic peptide derived from human Styk1 51-150/422 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | NOK Novel oncogene with kinase domain Protein kinase STYK1 Protein PK unique Protein PK-unique Serine/threonine/tyrosine kinase 1 Styk 1 STYK1 STYK1_HUMAN SuRTK 106 SuRTK106 Tyrosine protein kinase STYK1 Tyrosine-protein kinase STYK1. |
|---|---|
| Molecular Weight(MW) | 48 KDa |
| Cellular Localization | Membrane. |
| SwissProt ID | Q6J9G0 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Styk1) Polyclonal Antibody, Unconjugated (ER1917-32) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: Small intestine (Mouse) Lysate at 40 ug Primary: Anti-Styk1 (ER1917-32) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 48 kD| Positive Control | Widely expressed. Highly expressed in brain, placenta and prostate. Expressed in tumor cells such as hepatoma cells LO2, cervix carcinoma cells HeLa, ovary cancer cells Ho8910 and chronic myelogenous leukemia cells K562, but not in other tumor cells such as epidermoid carcinoma (A431). Undetectable in most normal lung tissues, widely expressed in lung cancers. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
