Application
Fig1: Protein: mouse heart lysate; Primary: rabbit Anti-Supervillin (ER1917-34) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size:248 kD Observed band size:248 kD| Product Name | Supervillin |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Archvillin p205/p250 SVIL_HUMAN. |
| Product description | Forms a high-affinity link between the actin cytoskeleton and the membrane. Isoform 1 (archvillin) is among the first costameric proteins to assemble during myogenesis and it contributes to myogenic membrane structure and differentiation. Appears to be involved in myosin II assembly. May modulate myosin II regulation through MLCK during cell spreading, an initial step in cell migration. May play a role in invadopodial function. Isoform 2 may be involved in modulation of focal adhesions. Supervillin-mediated down-regulation of focal adehesions involves binding to TRIP6 (By similarity). |
| Immunogen | KLH conjugated synthetic peptide derived from human Supervillin 51-150/2214 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | Archvillin p205/p250 SVIL_HUMAN. |
|---|---|
| Molecular Weight(MW) | 248 KDa |
| Cellular Localization | Cell membrane; peripheral membrane protein; cytoplasmic side. Cytoplasm; cytoskeleton. |
| SwissProt ID | O95425 |
|---|
Application
Fig1: Protein: mouse heart lysate; Primary: rabbit Anti-Supervillin (ER1917-34) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size:248 kD Observed band size:248 kD| Positive Control | Expressed in many tissues. Most abundant in muscle, bone marrow, thyroid gland and salivary gland. Isoform 1 (archvillin) is muscle specific. |
|---|---|
| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
