Application
Fig1: Sample: Placenta (Mouse) Lysate at 40 ug Primary: Anti-SUPT16H (ER1917-35) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 120 kD Observed band size: 120 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SUPT16H) Polyclonal Antibody, Unconjugated (ER1917-35) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | SUPT16H |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDC68 Chromatin specific transcription elongation factor 140 kDa subunit Chromatin-specific transcription elongation factor 140 kDa subunit Facilitates chromatin transcription complex subunit SPT16 FACT 140 kDa subunit FACT FACT complex subunit SPT16 FACTP140 FLJ10857 FLJ14010 hSPT16 SP16H_HUMAN Suppressor of Ty 16 homolog Supt16h. |
| Product description | Component of the FACT complex, a general chromatinfactor that acts to reorganize nucleosomes. The FACT complex isinvolved in multiple processes that require DNA as a template suchas mRNA elongation, DNA replication and DNA repair. Duringtranscription elongation the FACT complex acts as a histonechaperone that both destabilizes and restores nucleosomalstructure. It facilitates the passage of RNA polymerase II andtranscription by promoting the dissociation of one histone H2A-H2Bdimer from the nucleosome, then subsequently promotes thereestablishment of the nucleosome following the passage of RNApolymerase II. The FACT complex is probably also involved inphosphorylation of 'Ser-392' of p53/TP53 via its association withCK2 (casein kinase II). Also involved in vitamin D-coupledtranscription regulation via its association with the WINACcomplex, a chromatin-remodeling complex recruited by vitamin Dreceptor (VDR), which is required for the ligand-bound VDR-mediatedtransrepression of the CYP27B1 gene (By similarity). |
| Immunogen | KLH conjugated synthetic peptide derived from human SUPT16H/CDC68 601-700/1047 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | CDC68 Chromatin specific transcription elongation factor 140 kDa subunit Chromatin-specific transcription elongation factor 140 kDa subunit Facilitates chromatin transcription complex subunit SPT16 FACT 140 kDa subunit FACT FACT complex subunit SPT16 FACTP140 FLJ10857 FLJ14010 hSPT16 SP16H_HUMAN Suppressor of Ty 16 homolog Supt16h. |
|---|---|
| Molecular Weight(MW) | 120 KDa |
| Cellular Localization | Nucleus. Chromosome. Note=Colocalizes withRNA polymerase II on chromatin. Recruited to actively transcribedloci. |
| SwissProt ID | Q9Y5B9 |
|---|
Application
Fig1: Sample: Placenta (Mouse) Lysate at 40 ug Primary: Anti-SUPT16H (ER1917-35) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 120 kD Observed band size: 120 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SUPT16H) Polyclonal Antibody, Unconjugated (ER1917-35) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | Ubiquitous. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
