Application
Fig1: Sample: U937 Cell (Human) Lysate at 40 ug Primary: Anti-SWAP70(ER1917-38)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 69kD Observed band size: 63kD| Product Name | SWAP70 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KIAA0640 70kDa AV235546 FLJ39540 HSPC321 SWA 70 protein SWAP 70 SWAP switching B-cell complex 70kDa subunit SWAP-70 SWAP70 Switch associated protein 70 Switch-associated protein 70 SWP70_HUMAN. |
| Product description | Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion. |
| Immunogen | KLH conjugated synthetic peptide derived from human SWAP70 131-230/585 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | KIAA0640 70kDa AV235546 FLJ39540 HSPC321 SWA 70 protein SWAP 70 SWAP switching B-cell complex 70kDa subunit SWAP-70 SWAP70 Switch associated protein 70 Switch-associated protein 70 SWP70_HUMAN. |
|---|---|
| Molecular Weight(MW) | 69 KDa |
| Cellular Localization | Cytoplasm. Cell membrane. Nucleus. Cell projection > lamellipodium. In resting B-cells it is localized mainly in the cytoplasm and upon cell activation it is recruited to the plasma membrane and then translocates to the nucleus. In activated, class-switching B-cells it is associated with membrane IgG but not IgM. Localized to loose actin filament arrays located behind actively extending lamellipodia. |
| SwissProt ID | Q9UH65 |
|---|
Application
Fig1: Sample: U937 Cell (Human) Lysate at 40 ug Primary: Anti-SWAP70(ER1917-38)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 69kD Observed band size: 63kD| Positive Control | Expressed only in mature B-cells including those associated with mucosa-associated tissue and bronchus-associated tissue (PubMed:10681448). Widely expressed. Abundant in spleen, and fairly abundant in kidney, lung and liver. Also found in monocytes and macrophages (PubMed:12925760). |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
