Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Cerebellum (Mouse) Lysate at 40 ug Cerebral cortex (Mouse) Lysate at 40 ug Cerebrum(Rat) Lysate at 40 ug Primary: Anti-Synaptotagmin 1 (ER1917-41) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 53kD| Product Name | Synaptotagmin 1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DKFZp781D2042 P65 SVP65 Synaptotagmin I SYT1_HUMAN Synaptotagmin II Synaptotagmin IV Synaptotagmin V SYT 1 SYT SYT1 SYT2 SYT3 SYT4 SYT5 SytI. |
| Product description | May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca2+-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca2+-independent manner; these are neurexins, syntaxin and AP2. Plays a role in dendrite formation by melanocytes. The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse. |
| Immunogen | KLH conjugated synthetic peptide derived from human Synaptotagmin 1 165-260/422 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowRabbit |
| Concentration | 1mg/ml |
| Alternative Names | DKFZp781D2042
P65
SVP65 Synaptotagmin I SYT1_HUMAN Synaptotagmin II Synaptotagmin IV Synaptotagmin V SYT 1 SYT SYT1 SYT2 SYT3 SYT4 SYT5 SytI. |
|---|---|
| Molecular Weight(MW) | 48 KDa |
| Cellular Localization | Cytoplasmic, membrane. |
| SwissProt ID | P21579 |
|---|
Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Cerebellum (Mouse) Lysate at 40 ug Cerebral cortex (Mouse) Lysate at 40 ug Cerebrum(Rat) Lysate at 40 ug Primary: Anti-Synaptotagmin 1 (ER1917-41) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 53kD| Positive Control | Mouse Cerebrum Lysate, Mouse Cerebellum Lysate, Mouse Cerebral cortex Lysate, Rat Cerebrum Lysate. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
